Phylogenetic analyses of Pilosocereus (Cactaceae) inferred from plastid and nuclear sequences

Autor(es)

Calvente, Alice ; Moraes, Evandro M. ; Lavor, Pamela ; Bonatelli, Isabel A. S. ; Nacaguma, Pamela ; Versieux, Leonardo M. ; Taylor, Nigel P. ; Zappi, Daniela C.

• Tipo de documento: Artigo Científico
• Fonte: Botanical Journal of the Linnean Society; v. 183, n. 1, p. 25-38, JAN 1 2017.
• Citações Web of Science: 5

Resumo

Pilosocereus is a large genus of Cactaceae with 42 species of columnar cacti distributed in the Americas. In this work we investigate the phylogenetics and evolutionary history of Pilosocereus based in plastid and nuclear DNA sequences. We use phylogenetic trees obtained as a basis to analyse infrageneric relationships and to study the evolution of selected morphological characters and geographical distribution in the group. Thirty-three species of the genus were sampled and five molecular regions were selected, four non-coding intergenic spacers of plastid DNA (trnS-trnG, psbD-trnT, trnL-trnT, petL-psbE) and one nuclear low-copy gene (phytochrome C). The phylogenetic analyses obtained point to a paraphyletic Pilosocereus, with P. bohlei and P. gounellei emerging nested in a clade of outgroup species (i.e. other genera of Cereinae). However, the majority of species of the genus form one well supported clade (excluding P. bohlei and P. gounellei) corresponding mostly to Pilosocereus subgenus Pilosocereus. Evidence indicates that the ancestor of Pilosocereus subgenus Pilosocereus clade was a shrub with a straight floral tube occurring in Brazil and the ancestor of Pilosocereus subgenus Gounellea was a shrub with a curved floral tube also occurring in Brazil. The ancestral distribution in central and eastern Brazil resulted in the diversification of most lineages in the same area, whereas the P. leucocephalus clade was able to disperse through the Amazonian areas and diversify further north and reach Central and North America. (C) 2016 The Linnean Society of London, Botanical Journal of the Linnean Society, 2016 (AU)

• Processo FAPESP: 05/55200-8 - Estudo filogeográfico do grupo de espécies relacionadas a Pilosocereus aurisetus (Cactaceae) utilizando DNA microssatélite nuclear e seqüências nucleotídicas do genoma do cloroplasto
• Beneficiário: Evandro Marsola de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 08/50905-1 - Extração de DNA e amplificação de genes plastidiais de cactaceas do grupo de espécies Pilosocereus aurisetus
• Beneficiário: Isabel Aparecida da Silva Bonatelli
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/02186-6 - Análise da estrutura filogeográfica de Pilosocereus machrisii (Cactaceae) utilizando marcadores moleculares do genoma do cloroplasto(cpDNA)
• Beneficiário: Isabel Aparecida da Silva Bonatelli
• Modalidade de apoio: Bolsas no Brasil - Mestrado