Physiological and biochemical responses of Dolichos lablab L. to cadmium support its potential as a cadmium phytoremediator

Autor(es)

Souza, Lucas A. ; Piotto, Fernando A. ; Dourado, Manuella N. ; Schmidt, Daiana ; Franco, Monica R. ; Boaretto, Luis F. ; Tezotto, Tiago ; Ferreira, Renato R. ; Azevedo, Ricardo A.

• Tipo de documento: Artigo Científico
• Fonte: Journal of Soils and Sediments; v. 17, n. 5, p. 1413-1426, MAY 2017.
• Citações Web of Science: 5

Resumo

This study aims to investigate the response of a high biomass producer non-hyperaccumulator legume plant species, Dolichos lablab L., to cadmium (Cd) stress for phytoremediation process. Three individual experiments were carried out to assess physiological and biochemical parameters to support the use of this plant species as a phytoremediator. The first experiment was carried out in Cd-contaminated soil while the second and third experiments were conducted in sand in which Cd was applied to study biochemical responses. Analysis of mineral nutrition, phytoremediation parameters, antioxidant response, and protein identification by gel-based proteomics were performed. Good tolerance to Cd under moderate level of contamination was observed. Mineral nutrition was little affected, and phytoremediation index was satisfactory. Additionally, biochemical responses based on antioxidant enzyme analysis were well responsive in roots, reflecting the capacity of Cd stress attenuation in this organ. A proteomic analysis revealed positive regulation of root proteins involved in carbohydrate, amino acids, nitrogen metabolism, and abiotic/biotic stress response, which together may contribute to create a scenario to overcome Cd-induced stress. Based on the physiological and biochemical results, we concluded that D. lablab L. is suitable for phytoremediation/phytostabilization purposes. (AU)

• Processo FAPESP: 11/50368-9 - Efeito cádmio em bactérias isoladas de solo contaminado
• Beneficiário: Manuella Nóbrega Dourado Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21123-8 - Entendendo o metabolismo de lisina em milho de alta qualidade protéica (QPM) e em cevada geneticamente modificada
• Beneficiário: Daiana Schmidt
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/50167-0 - Estudo da resposta antioxidativa enzimatica, sintese de fitoquelatinas e do perfil proteico de dolichos lablab submetida a estresse por cadmo.
• Beneficiário: Lucas Anjos de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/54676-0 - Estresse oxidativo induzido por metais: novas abordagens
• Beneficiário: Ricardo Antunes de Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Temático