American cutaneous leishmaniasis: In situ immune response of patients with recent and late lesions

Autor(es)

Gomes, A. H. S. ; Martines, R. B. ; Kanamura, C. T. ; Barbo, M. L. P. ; Iglezias, S. D. ; Lauletta Lindoso, J. A. ; Pereira-Chioccola, V. L.

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. 39, n. 4 APR 2017.
• Citações Web of Science: 6

Resumo

TNF-alpha, IFN-gamma, IL-10, IL-17, CD68 and CD57 were evaluated in biopsies of patients with American cutaneous leishmaniasis living in Sorocaba, Brazil. The analyses were performed considering the time of lesions from 23 patients with recent lesions (Group I) and 19 patients with late lesions (Group II). All patients were infected with Leishmania (Viannia) braziliensis. Immunostaining cells for CD68, CD57, TNF-alpha, IFN-gamma, IL-10 and IL-17 were performed by immunohistochemistry. Except for CD68 and IL-17, the distribution of in situ for CD57, IL-10, TNF-alpha and IFN-gamma showed that patients with recent lesions expressed higher levels than those with late lesions. The comparison of cytokine expression/group showed that IL-10 was significantly higher than IL-17 and IFN-gamma (similar data were shown in IL-17 compared with TNF-alpha), suggesting an immunological balance between inflammatory-anti-inflammatory agents. This balance was similar for two groups of patients. In conclusion, these data suggested that (i) patients from Group I had recent lesions (in the beginning of chronic phase) compared to those from Group II and (ii) the modulation of inflammatory response in patients with recent American cutaneous leishmaniasis was correlated with IL-10 expression in skin lesions preventing the development of mucosal forms. The parasite treatment also prevented the evolution of severe forms. (AU)

• Processo FAPESP: 11/13939-8 - Estudo de um grupo de infecções fúngico-parasitárias visando a melhoria das técnicas de caracterização e diagnóstico imuno-molecular
• Beneficiário: Vera Lúcia Pereira Chioccola
• Modalidade de apoio: Auxílio à Pesquisa - Regular