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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Tissue factor-dependent coagulation activation by heme: A thromboelastometry study

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Autor(es):
de Souza, Gleice Regina ; Hounkpe, Bidossessi Wilfried ; Luz Fiusa, Maiara Marx ; Colella, Marina Pereira ; Annichino-Bizzacchi, Joyce M. ; Traina, Fabiola ; Costa, Fernando Ferreira ; De Paula, Erich Vinicius
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 12, n. 4 APR 24 2017.
Citações Web of Science: 1
Resumo

Heme has been characterized as potent trigger of inflammation. In hemostasis, although heme has been shown to both induce and inhibit different compartments of hemostasis, its net effect on the hemostatic balance, and the biological relevance of these effects remain to be determined. Herein we evaluated the effect of heme on hemostasis using a global assay able to generate clinically relevant data in several other complex hemostatic diseases. Citrated whole blood samples from healthy participants were stimulated by heme or vehicle and incubated for 4h at 37 degrees C. Rotational thromboelastometry was immediately performed. The participation of tissue factor in coagulation activation was evaluated using inhibitory antibody. Heme was able of inducing ex vivo coagulation activation in whole blood, affecting predominantly parameters associated with the initial phases of clot formation. This activation effect was at least partially dependent on hematopoietic tissue factor, since the effects of heme were partially abrogated by the inhibition of human tissue factor. In conclusion, using a global hemostasis assay, our study confirmed that heme is able to activate coagulation in whole blood, in a tissue factor-dependent way. These findings could explain the disturbance in hemostatic balance observed in conditions associated with the release of heme such as sickle cell disease. (AU)

Processo FAPESP: 15/24666-3 - Investigação de novos mecanismos fisiopatológicos de hipercoagulabilidade na doença falciforme
Beneficiário:Bidossessi Wilfried Hounkpe
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/09319-0 - Exploração de novos mecanismos fisiopatológicos da doença do enxerto contra o hospedeiro aguda intestinal relacionados à quebra da barreira epitelial intestinal e alterações em junções "tight"
Beneficiário:Erich Vinicius de Paula
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/00984-3 - Doenças dos glóbulos vermelhos: fisiopatologia e novas abordagens terapêuticas
Beneficiário:Fernando Ferreira Costa
Linha de fomento: Auxílio à Pesquisa - Temático