Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro

Autor(es)

Ambrogi, M. ; Dall'Acqua, P. C. ; Rocha-Frigoni, N. A. S. ; Leao, B. C. S. ; Mingoti, G. Z.

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 52, n. 3, p. 409-421, JUN 2017.
• Citações Web of Science: 4

Resumo

Contents We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules {[}foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18hr. The oocytes in the control groups were cultured (standard IVM) for 24hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox). The intracellular concentrations of reactive oxygen species (ROS) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group (FCS: 0.62 and BSA: 0.66 vs. Control FCS: 1.00, p<.05; and Transp: 0.58 and Transp Antiox: 0.70 vs. Control FCS: 1.00, p<.05). After IVM, the mitochondrial membrane potentials of the transported oocytes were lower than those of the non-transported oocytes (FCS: 0.41 and BSA: 0.57 vs. Control FCS: 1.00, p<.05; and Transp: 0.48 and Transp Antiox: 0.51 vs. Control FCS: 1.00 and Control Antiox: 0.84, p<.05). The blastocyst formation rates (36.9% average) and the re-expansion rates of vitrified-warmed blastocysts (53%, average) were unaffected (p>.05) by the treatments. In conclusion, supplementing the medium in which bovine oocytes are transported with antioxidants or different macromolecules did not affect their in vitro production of embryos or their cryotolerance. (AU)

• Processo FAPESP: 15/06733-5 - Avaliação das características seminais em tourinhos da raça Nelore classificados para consumo alimentar residual (CAR) e suplementados com fonte de ácidos graxos poliinsaturados em regime de pastagem
• Beneficiário: Gisele Zoccal Mingoti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07382-6 - Adição de ácido linolênico e L-carnitina na maturação oocitária: efeitos sobre o metabolismo celular, potencial de desenvolvimento e criotolerância de embriões bovinos produzidos in vitro
• Beneficiário: Gisele Zoccal Mingoti
• Modalidade de apoio: Auxílio à Pesquisa - Regular