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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro

Full text
Author(s):
Ambrogi, M. ; Dall'Acqua, P. C. ; Rocha-Frigoni, N. A. S. ; Leao, B. C. S. ; Mingoti, G. Z.
Total Authors: 5
Document type: Journal article
Source: REPRODUCTION IN DOMESTIC ANIMALS; v. 52, n. 3, p. 409-421, JUN 2017.
Web of Science Citations: 4
Abstract

Contents We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules {[}foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18hr. The oocytes in the control groups were cultured (standard IVM) for 24hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox). The intracellular concentrations of reactive oxygen species (ROS) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group (FCS: 0.62 and BSA: 0.66 vs. Control FCS: 1.00, p<.05; and Transp: 0.58 and Transp Antiox: 0.70 vs. Control FCS: 1.00, p<.05). After IVM, the mitochondrial membrane potentials of the transported oocytes were lower than those of the non-transported oocytes (FCS: 0.41 and BSA: 0.57 vs. Control FCS: 1.00, p<.05; and Transp: 0.48 and Transp Antiox: 0.51 vs. Control FCS: 1.00 and Control Antiox: 0.84, p<.05). The blastocyst formation rates (36.9% average) and the re-expansion rates of vitrified-warmed blastocysts (53%, average) were unaffected (p>.05) by the treatments. In conclusion, supplementing the medium in which bovine oocytes are transported with antioxidants or different macromolecules did not affect their in vitro production of embryos or their cryotolerance. (AU)

FAPESP's process: 15/06733-5 - Evaluation of seminal characteristics in Nellore bulls selected for residual feed intake and supplemented with polyunsaturated fatty acids in pasture conditions
Grantee:Gisele Zoccal Mingoti
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07382-6 - Supplementation with linolenic acid and L-carnitine during oocyte maturation: effects on cellular metabolism, developmental potential and cryotolerance of bovine embryos produced in vitro
Grantee:Gisele Zoccal Mingoti
Support Opportunities: Regular Research Grants