FBXO25 regulates MAPK signaling pathway through inhibition of ERK1/2 phosphorylation

Autor(es)

Teixeira, Felipe R. ; Manfiolli, Adriana O. ; Vieira, Nichelle A. ; Medeiros, Ana Carla ; Coelho, Priscila de O. ; Guimaraes, Dimitrius Santiago ; Schechtman, Deborah ; Gomes, Marcelo D.

• Tipo de documento: Artigo Científico
• Fonte: Archives of Biochemistry and Biophysics; v. 621, p. 38-45, MAY 1 2017.
• Citações Web of Science: 2

Resumo

The FBXO25 mediates degradation of ELK-1 and thus inhibits transcriptional activation of immediate early genes (iEG). Here we show that FBXO25 regulates yet another node of this signaling pathway, by decreasing MAPK/ERK activity. We show that induction of FBXO25 reduced ERK1/2 phosphorylation independently of MEK1/2. Accordingly, in HAP1 FBXO25 knockout cells (FBXO25(KO)), we observed that upon PMA treatment ERK1/2 was more active than in parental cells. An increase in cell proliferation under receptor mediated activation of the ERK signaling pathway in FBXO25(KO) cells was also observed. Taken together we show that FBXO25 functions as a negative regulator of MAPK signaling though the reduction of ERK1/2 activation. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/21891-5 - Produção e análise funcional de células neuronais humanas geneticamente modificadas para regular a expressão da ubiquitina ligase FBXO25
• Beneficiário: Adriana Oliveira Manfiolli
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/10898-7 - Triagem do tipo high-throughput de bibliotecas de RNAi para identificar novos reguladores das ERKs
• Beneficiário: Marcelo Damário Gomes
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/16464-8 - Utilização de microarranjos de proteínas (protoarrays) para identificação de substratos da E3 ubiquitina ligase SCF1 (FBXO25)
• Beneficiário: Felipe Roberti Teixeira
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado