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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

FBXO25 regulates MAPK signaling pathway through inhibition of ERK1/2 phosphorylation

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Author(s):
Teixeira, Felipe R. ; Manfiolli, Adriana O. ; Vieira, Nichelle A. ; Medeiros, Ana Carla ; Coelho, Priscila de O. ; Guimaraes, Dimitrius Santiago ; Schechtman, Deborah ; Gomes, Marcelo D.
Total Authors: 8
Document type: Journal article
Source: Archives of Biochemistry and Biophysics; v. 621, p. 38-45, MAY 1 2017.
Web of Science Citations: 2
Abstract

The FBXO25 mediates degradation of ELK-1 and thus inhibits transcriptional activation of immediate early genes (iEG). Here we show that FBXO25 regulates yet another node of this signaling pathway, by decreasing MAPK/ERK activity. We show that induction of FBXO25 reduced ERK1/2 phosphorylation independently of MEK1/2. Accordingly, in HAP1 FBXO25 knockout cells (FBXO25(KO)), we observed that upon PMA treatment ERK1/2 was more active than in parental cells. An increase in cell proliferation under receptor mediated activation of the ERK signaling pathway in FBXO25(KO) cells was also observed. Taken together we show that FBXO25 functions as a negative regulator of MAPK signaling though the reduction of ERK1/2 activation. (C) 2017 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 14/10898-7 - High-throughput RNAi library screening to identify novel regulators of ERKs
Grantee:Marcelo Damário Gomes
Support type: Regular Research Grants
FAPESP's process: 10/16464-8 - Application of protein microarray to identify substrates of SCF1 (FBXO25) E3 ubiquitin ligase
Grantee:Felipe Roberti Teixeira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/21891-5 - PRODUCTION AND FUNCTIONAL ANALYSIS OF HUMAN NEURONAL CELLS GENETICALLY MODIFIED TO REGULATE THE UBIQUITIN LIGASE FBXO25 EXPRESSION
Grantee:Adriana Oliveira Manfiolli
Support type: Scholarships in Brazil - Post-Doctorate