Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry

Autor(es):Mostrar menos -

Quini, Caio C. ; Prospero, Andre G. ; Calabresi, Marcos F. F. ; Moretto, Gustavo M. ; Zufelato, Nicholas ; Krishnan, Sunil ; Pina, Diana R. ; Oliveira, Ricardo B. ; Baffa, Oswaldo ; Bakuzis, Andris F. ; Miranda, Jose R. A.

• Tipo de documento: Artigo Científico
• Fonte: Nanomedicine-Nanotechnology Biology and Medicine; v. 13, n. 4, p. 1519-1529, MAY 2017.
• Citações Web of Science: 9

Resumo

We describe the development of a joint in vivo/ex vivo protocol to monitor magnetic nanoparticles in animal models. Alternating current biosusceptometry (ACB) enables the assessment of magnetic nanoparticle accumulation, followed by quantitative analysis of concentrations in organs of interest. We present a study of real-time liver accumulation, followed by the assessment of sequential biodistribution using the same technique. For quantification, we validated our results by comparing all of the data with electron spin resonance (ESR). The ACB had viable temporal resolution and accuracy to differentiate temporal parameters of liver accumulation, caused by vasculature extravasation and macrophages action. The biodistribution experiment showed different uptake profiles for different doses and injection protocols. Comparisons with the ESR system indicated a correlation index of 0.993. We present the ACB system as an accessible and versatile tool to monitor magnetic nanoparticles, allowing in vivo and real-time evaluations of distribution and quantitative assessments of particle concentrations. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 10/07639-9 - Motilidade gastrintestinal: investigações clínicas, fisiopatológicas e biofarmacêuticas
• Beneficiário: Ricardo Brandt de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/20842-6 - Avaliação da influência do câncer de pâncreas nas funções metabólicas hepática e renal
• Beneficiário: Caio César Quini
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/18696-6 - Novas aplicações da BAC empregando nano partículas magnéticas
• Beneficiário: Caio César Quini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/14914-0 - Biosusceptometria AC aplicada à detecção de nanopartículas magnéticas para diagnóstico e terapia alvo
• Beneficiário: Caio César Quini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado