Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes

Autor(es)

del Collado, Maite ; da Silveira, Juliano Coelho ; Sangalli, Juliano Rodrigues ; Andrade, Gabriella Mamede ; da Silva Sousa, Leticia Rabello ; Silva, Luciano Andrade ; Meirelles, Flavio Vieira ; Perecin, Felipe

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, JUN 1 2017.
• Citações Web of Science: 14

Resumo

Oocytes that undergo in vitro maturation (IVM) are metabolically abnormal and accumulate excess lipid content. However, the mechanism of lipid accumulation and the role of cumulus cells in this process are unclear. Recently, it was shown that fatty acid binding proteins (FABPs) performed intra- and extracellular fatty acid transport. We postulated that FABP3 might be responsible for fatty acid transport from cumulus cells to the oocytes via transzonal projections (TZPs) during IVM. Transcript and protein levels of FABP3 were analyzed in both in vivo- and in vitro-matured cumulus-oocyte-complexes and were increased in IVM samples. Further analysis showed increased lipid content in oocytes and cumulus cells in IVM samples compared to in vivo- derived. We therefore speculated that altered traffic of fatty acids via FABP3 during IVM was the mechanism leading to the excess of lipids accumulated within IVM oocytes. Furthermore, we demonstrated an increase in FABP3 levels and lipid content during the first 9 h of IVM, further strengthening the possibility of fatty acid transport via FABP3 and TZPs. Additionally, disruptions of TZPs during IVM decreased lipid accumulation in oocytes. Our results shed light on a possible mechanism involving FABP3 and TZPs that causes excess lipid accumulation in oocytes during IVM. (AU)

• Processo FAPESP: 14/03281-3 - Dinâmica lipídico-mitocondrial e moduladores do metabolismo oxidativo em embriões bovinos produzidos in vitro
• Beneficiário: Maite Del Collado Barrondo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/26056-8 - Quantificação de proteínas relacionadas ao metabolismo lipídico nas células do cumulus durante a maturação in vivo e in vitro de oócitos bovinos
• Beneficiário: Letícia Rabello da Silva Sousa
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/21034-3 - Rotas intracelulares e intercelulares durante a foliculogênese e maturação oocitária in vivo e in vitro
• Beneficiário: Felipe Perecin
• Modalidade de apoio: Auxílio à Pesquisa - Regular