Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers

Autor(es)

Luz, Maria Claudia de B. ; Perez, Matheus M. ; Azzalis, Ligia A. ; Sousa, Luiz Vinicius de A. ; Adami, Fernando ; Fonseca, Fernando L. A. ; Alves, Beatriz da C. A.

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 18, n. 4 APR 2017.
• Citações Web of Science: 3

Resumo

Background: Patients with breast cancer-the deadliest cancer among women-are at constant risk of developing metastasis. Oxidative stress and hypoxia are common feature of tumor cells that can proliferate even in a resultant metabolic acidosis. Despite the low extracellular pH, intracellular pH of tumor cells remains relatively normal, or even more alkaline due to the action of a membrane protein family known as monocarboxylate transporters (MCTs). The objective of this study was to verify the diagnostic and prognostic value of MCT1, MCT4 and CD147 in tumor and peripheral blood samples of patients with breast cancer undergoing chemotherapic treatment. Methods: Differential expression of MCT1, MCT4 and CD147 obtained by qPCR was determined by 2(-Delta Delta Cq) method between biological samples (tumor and serial samples of peripheral) of patients (n = 125) and healthy women (n = 25). Results: tumor samples with higher histological grades have shown higher expression of these markers; this higher expression was also observed in blood samples obtained at diagnosis of patients when compared to healthy women and in patients with positive progression of the disease (metastasis development). Conclusion: markers studied here could be a promising strategy in routine laboratory evaluations as breast cancer diagnosis and prognosis. (AU)

• Processo FAPESP: 14/17693-1 - Avaliação do valor prognóstico de transportadores de monocarboxilatos (MCT) em pacientes com câncer de mama
• Beneficiário: Beatriz da Costa Aguiar Alves Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular