Formation of multi-species biofilms by Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus isolated from ricotta processing and effectiveness of chemical sanitation procedures

Autor(es)

Fernandes, Meg da Silva ; Coelho Alvares, Ana Carolina ; Martins Manoel, Joao Gabriel ; Ramires Esper, Luciana Maria ; Kabuki, Dirce Yorika ; Kuaye, Arnaldo Yoshiteru

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL DAIRY JOURNAL; v. 72, p. 23-28, SEP 2017.
• Citações Web of Science: 6

Resumo

The formation of multi-species biofilms by Enterococcus faecalis, Enterococcus faecium, and Bacillus cereus and the effectiveness of sanitation procedures in their removal were evaluated. Biofilms were grown on stainless steel at 7, 25, and 39 degrees C for 8 days. At 7 degrees C the microorganisms did not form biofilm. After 8 days at 25 and 39 degrees C the counts of Enterococcus sp. were above 8 log cfu cm(-2), while the counts of B. cereus vegetative cells were <0.4 log cfu cm(-2). However, there was an increase in the counts of B. cereus spores (above 4 log cfu cm(-2)). Anionic-tensioactive cleaning complemented with another procedure (acid-cleaning, disinfection or acid-cleaning + disinfection) eliminated the multi-species biofilms formed after 1 and 8 days of contact at 25 and 39 degrees C. Peracetic acid was the most effective disinfectant and sodium hypochlorite disinfectant the least when only applying the disinfection step directly to the biofilm. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/10507-7 - FORMAÇÃO DE BIOFILMES, PATOGENICIDADE E QUORUM-SENSING EM CEPAS DE E. faecium E E. faecalis ISOLADAS NO PROCESSAMENTO DE RICOTA.
• Beneficiário: Meg da Silva Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado