Formation of multi-species biofilms by Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus isolated from ricotta processing and effectiveness of chemical sanitation procedures

Author(s)

Fernandes, Meg da Silva ; Coelho Alvares, Ana Carolina ; Martins Manoel, Joao Gabriel ; Ramires Esper, Luciana Maria ; Kabuki, Dirce Yorika ; Kuaye, Arnaldo Yoshiteru

• Document type: Journal article
• Source: INTERNATIONAL DAIRY JOURNAL; v. 72, p. 23-28, SEP 2017.
• Web of Science Citations: 6

Abstract

The formation of multi-species biofilms by Enterococcus faecalis, Enterococcus faecium, and Bacillus cereus and the effectiveness of sanitation procedures in their removal were evaluated. Biofilms were grown on stainless steel at 7, 25, and 39 degrees C for 8 days. At 7 degrees C the microorganisms did not form biofilm. After 8 days at 25 and 39 degrees C the counts of Enterococcus sp. were above 8 log cfu cm(-2), while the counts of B. cereus vegetative cells were <0.4 log cfu cm(-2). However, there was an increase in the counts of B. cereus spores (above 4 log cfu cm(-2)). Anionic-tensioactive cleaning complemented with another procedure (acid-cleaning, disinfection or acid-cleaning + disinfection) eliminated the multi-species biofilms formed after 1 and 8 days of contact at 25 and 39 degrees C. Peracetic acid was the most effective disinfectant and sodium hypochlorite disinfectant the least when only applying the disinfection step directly to the biofilm. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 10/10507-7 - BIOFILM FORMATION, PATHOGENICITY AND QUORUM-SENSING IN STRAINS OF E. faecium AND E. faecalis ISOLATED IN PROCESSING OF RICOTTA.
• Grantee: Meg da Silva Fernandes
• Support Opportunities: Scholarships in Brazil - Doctorate