Autor(es)

Santana Gondim, Antonio Nei ; Lara, Aline ; Santos-Miranda, Artur ; Roman-Campos, Danilo ; Lauton-Santos, Sandra ; Rodrigues Menezes-Filho, Jose Evaldo ; Lins de Vasconcelos, Carla Maria ; Conde-Garcia, Eduardo Antonio ; Guatimosim, Silvia ; Cruz, Jader S.

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Pharmacology; v. 807, p. 56-63, JUL 15 2017.
• Citações Web of Science: 4

Resumo

(-)-Terpinen-4-ol is a naturally occurring plant monoterpene and has been shown to have a plethora of biological activities. The objective of this study was to investigate the effects of (-)-terpinen-4-ol on the rat heart, a key player in the control and maintenance of arterial blood pressure. The effects of (-)-terpinen-4-ol on the rat heart were investigated using isolated left atrium isometric force measurements, in vivo electrocardiogram (ECG) recordings, patch clamp technique, and confocal microscopy. It was observed that (-)-terpinen-4-ol reduced contraction force in an isolated left atrium at millimolar concentrations. Conversely, it induced a positive inotropic effect and extrasystoles at micromolar concentrations, suggesting that (-)-terpinen-4-ol may have arrhythmogenic activity on cardiac tissue. In anaesthetized animals, (-)-terpinen-4-ol also elicited rhythm disturbance, such as supraventricular tachycardia and atrioventricular block. To investigate the cellular mechanism underlying the dual effect of (-)-terpinen-4-ol on heart muscle, experiments were performed on isolated ventricular cardiomyocytes to determine the effect of (-)-terpinen-4-ol on L-type Ca2+ currents, Ca2+ sparks, and Ca2+ transients. The arrhythmogenic activity of (-)-terpinen-4-ol in vitro and in vivo may be explained by its effect on intracellular Ca2+ handling. Taken together, our data suggest that (-)-terpinen-4-ol has cardiac arrhythmogenic activity. (AU)

• Processo FAPESP: 14/09861-1 - Papel da corrente tardia de sódio nas arritmias cardíacas herdadas e adquiridas: da biofísica à procura de novos alvos terapêuticos
• Beneficiário: Danilo Roman Campos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores