Information Visualization and Feature Selection Methods Applied to Detect Gliadin in Gluten-Containing Foodstuff with a Microfluidic Electronic Tongue

Autor(es)

Daikuzono, Cristiane M. ; Shimizu, Flavio M. ; Manzoli, Alexandra ; Riul, Jr., Antonio ; Piazzetta, Maria H. O. ; Gobbi, Angelo L. ; Correa, Daniel S. ; Paulovich, Fernando V. ; Oliveira, Jr., Osvaldo N.

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 9, n. 23, p. 19646-19652, JUN 14 2017.
• Citações Web of Science: 13

Resumo

The fast growth of celiac disease diagnosis has sparked the production of gluten-free food and the search for reliable methods to detect gluten in foodstuff. In this paper, we report on a microfluidic electronic tongue (e-tongue) capable of detecting trace amounts of gliadin, a protein of gluten, down to 0.005 mg kg(-1) in ethanol solutions, and distinguishing between gluten-free and gluten-containing foodstuff. In some cases, it is even possible to determine whether gluten-free foodstuff has been contaminated with gliadin. That was made possible with an e-tongue comprising four sensing units, three of which made of layer-by-layer (LbL) films of semiconducting polymers deposited onto gold interdigitated electrodes placed inside microchannels Impedance spectroscopy was employed as the principle of detection, and the electrical capacitance data collected with the e-tongue were treated with information visualization techniques with feature selection for optimizing performance. The sensing units are disposable to avoid cross-contamination as gliadin adsorbs irreversibly onto the LbL films according to polarization modulated infrared reflection absorption spectroscopy (PM-IRRAS) analysis. Small amounts of material are required to produce the nanostructured films, however, and the e-tongue methodology is promising for low-cost, reliable detection of gliadin and other gluten constituents in foodstuff. (AU)

• Processo FAPESP: 14/03691-7 - Fabricação de dispositivos microfluídicos visando aplicações em agricultura de precisão e bioenergia
• Beneficiário: Antonio Riul Júnior
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/15543-7 - Biossensores para detectar Escherichia coli usando o conceito de línguas eletrônicas
• Beneficiário: Flavio Makoto Shimizu
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/16789-5 - Materiais nanoestruturados híbridos baseados em polímeros conjugados e partículas metálicas aplicados em sensores
• Beneficiário: Daniel Souza Corrêa
• Linha de fomento: Auxílio à Pesquisa - Regular