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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Information Visualization and Feature Selection Methods Applied to Detect Gliadin in Gluten-Containing Foodstuff with a Microfluidic Electronic Tongue

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Daikuzono, Cristiane M. ; Shimizu, Flavio M. ; Manzoli, Alexandra ; Riul, Jr., Antonio ; Piazzetta, Maria H. O. ; Gobbi, Angelo L. ; Correa, Daniel S. ; Paulovich, Fernando V. ; Oliveira, Jr., Osvaldo N.
Total Authors: 9
Document type: Journal article
Source: ACS APPLIED MATERIALS & INTERFACES; v. 9, n. 23, p. 19646-19652, JUN 14 2017.
Web of Science Citations: 13

The fast growth of celiac disease diagnosis has sparked the production of gluten-free food and the search for reliable methods to detect gluten in foodstuff. In this paper, we report on a microfluidic electronic tongue (e-tongue) capable of detecting trace amounts of gliadin, a protein of gluten, down to 0.005 mg kg(-1) in ethanol solutions, and distinguishing between gluten-free and gluten-containing foodstuff. In some cases, it is even possible to determine whether gluten-free foodstuff has been contaminated with gliadin. That was made possible with an e-tongue comprising four sensing units, three of which made of layer-by-layer (LbL) films of semiconducting polymers deposited onto gold interdigitated electrodes placed inside microchannels Impedance spectroscopy was employed as the principle of detection, and the electrical capacitance data collected with the e-tongue were treated with information visualization techniques with feature selection for optimizing performance. The sensing units are disposable to avoid cross-contamination as gliadin adsorbs irreversibly onto the LbL films according to polarization modulated infrared reflection absorption spectroscopy (PM-IRRAS) analysis. Small amounts of material are required to produce the nanostructured films, however, and the e-tongue methodology is promising for low-cost, reliable detection of gliadin and other gluten constituents in foodstuff. (AU)

FAPESP's process: 14/03691-7 - Fabrication of microfluidic devices envisanging aplications in precision agriculture and bioenergy
Grantee:Antonio Riul Júnior
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 12/15543-7 - Biosensors to detect Escherichia coli using the concept of an extended electronic tongue
Grantee:Flavio Makoto Shimizu
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/16789-5 - Hybrid nanostructured materials based on conjugated polymers and metallic nanoparticles for sensor applications
Grantee:Daniel Souza Corrêa
Support type: Regular Research Grants
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support type: Research Projects - Thematic Grants