| Full text | |
| Author(s): |
Daikuzono, Cristiane M.
;
Shimizu, Flavio M.
;
Manzoli, Alexandra
;
Riul, Jr., Antonio
;
Piazzetta, Maria H. O.
;
Gobbi, Angelo L.
;
Correa, Daniel S.
;
Paulovich, Fernando V.
;
Oliveira, Jr., Osvaldo N.
Total Authors: 9
|
| Document type: | Journal article |
| Source: | ACS APPLIED MATERIALS & INTERFACES; v. 9, n. 23, p. 19646-19652, JUN 14 2017. |
| Web of Science Citations: | 13 |
| Abstract | |
The fast growth of celiac disease diagnosis has sparked the production of gluten-free food and the search for reliable methods to detect gluten in foodstuff. In this paper, we report on a microfluidic electronic tongue (e-tongue) capable of detecting trace amounts of gliadin, a protein of gluten, down to 0.005 mg kg(-1) in ethanol solutions, and distinguishing between gluten-free and gluten-containing foodstuff. In some cases, it is even possible to determine whether gluten-free foodstuff has been contaminated with gliadin. That was made possible with an e-tongue comprising four sensing units, three of which made of layer-by-layer (LbL) films of semiconducting polymers deposited onto gold interdigitated electrodes placed inside microchannels Impedance spectroscopy was employed as the principle of detection, and the electrical capacitance data collected with the e-tongue were treated with information visualization techniques with feature selection for optimizing performance. The sensing units are disposable to avoid cross-contamination as gliadin adsorbs irreversibly onto the LbL films according to polarization modulated infrared reflection absorption spectroscopy (PM-IRRAS) analysis. Small amounts of material are required to produce the nanostructured films, however, and the e-tongue methodology is promising for low-cost, reliable detection of gliadin and other gluten constituents in foodstuff. (AU) | |
| FAPESP's process: | 14/16789-5 - Hybrid nanostructured materials based on conjugated polymers and metallic nanoparticles for sensor applications |
| Grantee: | Daniel Souza Corrêa |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/14262-7 - Nanostructured films from biologically-relevant materials |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/03691-7 - Fabrication of microfluidic devices envisanging aplications in precision agriculture and bioenergy |
| Grantee: | Antonio Riul Júnior |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 12/15543-7 - Biosensors to detect Escherichia coli using the concept of an extended electronic tongue |
| Grantee: | FLAVIO MAKOTO SHIMIZU |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |