A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry

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da Silva, Michelli Massaroli ; Andrade, Moacir dos Santos ; Bauermeister, Anelize ; Merfa, Marcus Vinicius ; Forim, Moacir Rossi ; Fernandes, Joao Batista ; Vieira, Paulo Cezar ; das Gracas Fernandes da Silva, Maria Fatima ; Lopes, Norberto Peporine ; Machado, Marcos Antonio ; de Souza, Alessandra Alves

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 22, n. 6 JUN 2017.
• Citações Web of Science: 4

Resumo

Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown. (AU)

• Processo FAPESP: 16/16117-2 - Estudo químico de plantas, fungos e bactérias: uma contribuição ao controle biorracional de pragas agrícolas
• Beneficiário: Maria Fátima das Graças Fernandes da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/25299-6 - Estudos integrados para o controle de formigas cortadeiras
• Beneficiário: João Batista Fernandes
• Modalidade de apoio: Auxílio à Pesquisa - Temático