Methylene Violet 3 RAX Dye as a New Reagent for the Determination of Nitrite in Cured Meats and Vegetables

Autor(es)

Caroline O. da Rocha ^[1] ; João Flávio S. Petruci ^[2] ; Arnaldo A. Cardoso ^[3]

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista. Departamento de Química Analítica - Brasil

^[2] Universidade Estadual Paulista. Departamento de Química Analítica - Brasil

^[3] Universidade Estadual Paulista. Departamento de Química Analítica - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 28, n. 8, p. 1528-1533, 2017-08-00.

Resumo

In this contribution we propose, for the first time, the use of Methylene Violet 3 RAX dye for the detection and determination of nitrite in cured meats and vegetables. Quantification is based on the decrease in absorbance of the dye, which is proportional to the nitrite concentration. The proposed method presents linear response (r > 0.99) in the range from 1.8 to 9 µmol L-1 of nitrite, with a limit of detection of 0.14 µmol L-1. Satisfactory accuracy with the official Association of Official Agricultural Chemists (AOAC) method was achieved, and the method was successfully applied to a wide range of cured meats and vegetables. The proposed method offers similar or superior performance to other optical methods used for the determination of nitrite in different matrices, with advantage in terms of reduction of chemical waste for each analysis, by minimizing amounts of reagents and products. (AU)

• Processo FAPESP: 13/22995-4 - Sensor luminescente para determinação in situ de compostos reduzidos de enxofre presentes em matrizes gasosas utilizando compostos de paládio
• Beneficiário: João Flávio da Silveira Petruci
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado