High-throughput continuous production of liposomes using hydrodynamic flow-focusing microfluidic devices

Autor(es)

Michelon, Mariano ; Bernardes Oliveira, Davi Rocha ; Furtado, Guilherme de Figueiredo ; de la Torre, Lucimara Gaziola ; Cunha, Rosiane Lopes

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 156, p. 349-357, AUG 1 2017.
• Citações Web of Science: 10

Resumo

The microfluidic hydrodynamic flow-focusing is a simple technique for nanoscale liposome formation that provides several advantages compared to the traditional manufacturing techniques. This work aimed to perform a systematic study of the liposome formation using planar microfluidic devices with different channel aspect-ratios, as an alternative to enhance the throughput of liposome synthesis. In general, liposomes with a low polydispersity and a precise control of the size were successfully produced from alteration of the flow rate ratio and channel aspect-ratio. The higher aspect-ratio enabled the most rapid generation of liposomes with similar diameter and significant lower polydispersity index than the obtained by other batch technique. Besides, beta-carotene was successfully incorporated into liposomes with efficiency of approximately 60% and the incorporation ability was not specific to a choice of microfluidic device aspect-ratio. The results suggest that the use of microfluidic devices could be employed for liposome production with a possible advantage to minimize the degree of parallelization of processes. These results demonstrate the potential technical feasibility of microfluidic processes for future industrial applications. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/54137-1 - EMUS: aquisição de um analisador de distribuição de tamanho de partículas, um medidor de potencial zeta, um spray dryer e um sistema de cromatografia líquida de ultra alta pressão / espectrômetro de massa
• Beneficiário: Miriam Dupas Hubinger
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 07/54520-4 - Estudo do processo de hidrolise enzimatica de carne de frango, microencapsulacao e secagem por spray dryer.
• Beneficiário: Miriam Dupas Hubinger
• Modalidade de apoio: Auxílio à Pesquisa - Regular