Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil

Autor(es)

Juliana Helena Chávez ; Vinicius Pinho dos Reis ; Jaqueline Raymondi Silva ; Helen Julie Laure ; José Cesar Rosa ; Benedito Antônio Lopes da Fonseca ; Luiz Tadeu Moraes Figueiredo

• Tipo de documento: Artigo Científico
• Fonte: Revista da Sociedade Brasileira de Medicina Tropical; v. 46, n. 1, p. -, Jan. 2013.

Resumo

INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil. (AU)

• Processo FAPESP: 06/01179-0 - Produção e aplicação diagnóstica de polipeptídeos recombinantes derivados da proteína e de Flavivirus do complexo da Encefalite Japonesa
• Beneficiário: Juliana Helena Chávez
• Linha de fomento: Bolsas no Brasil - Doutorado