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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil

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Author(s):
Juliana Helena Chávez [1] ; Vinicius Pinho dos Reis [2] ; Jaqueline Raymondi Silva [3] ; Helen Julie Laure ; José Cesar Rosa ; Benedito Antônio Lopes da Fonseca [6] ; Luiz Tadeu Moraes Figueiredo [7]
Total Authors: 7
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia
[2] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia
[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia
[6] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia
[7] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia
Total Affiliations: 7
Document type: Journal article
Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 46, n. 1, p. 97-99, 2013-02-00.
Abstract

INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil. (AU)