Low doses of IgG from atopic individuals can modulate in vitro IFN-gamma production by human intra-thymic TCD4 and TCD8 cells: An IVIg comparative approach

Autor(es)

da Ressureicao Sgnotto, Fabio ; Garcia de Oliveira, Marilia ; de Lima Lira, Aline Aparecida ; Bento-de-Souza, Luciana ; da Silva Duarte, Alberto Jose ; Russo Victor, Jefferson

• Tipo de documento: Artigo Científico
• Fonte: HUMAN VACCINES & IMMUNOTHERAPEUTICS; v. 13, n. 7, p. 1563-1572, 2017.
• Citações Web of Science: 5

Resumo

The regulatory effect of allergic responses induced by IgG antibodies on human intra-thymic cells has not been reported in the literature. The aim of this study was to evaluate the possible differential effect of purified IgG from atopic and non-atopic individuals on human intra-thymic T cell cytokine production. Thymic tissues were obtained from 14 patients who were less than 7 d old. Additionally, blood samples were collected from atopic and non-atopic volunteers. Thymocytes and peripheral blood mononuclear cells were cultured with purified atopic or non-atopic IgG, and intracellular cytokine production was assessed. Purified IgG did not influence the frequency or viability of human intra-thymic T cells. Purified non-atopic IgG induced greater IFN-gamma production by intra-thymic CD4+CD8+ T cells than did the mock treatment and atopic IgG. A similar effect of purified non-atopic IgG on TCD8 cells was observed compared with the mock treatment. Atopic IgG inhibited IFN-gamma and TGF-beta production by intra-thymic TCD4 cells. Treatment with intravenous immunoglobulin resulted in intermediate levels of IFN-gamma and TGF-beta in intra-thymic TCD4 cells compared with treatment with atopic and non-atopic IgG. Peripheral TCD4 cells from non-atopic individuals produced IFN-gamma only in response to atopic IgG. This report describes novel evidence revealing that IgG from atopic individuals may influence intracellular IFN-gamma production by intra-thymic T cells in a manner that may favor allergy development. (AU)

• Processo FAPESP: 15/17256-3 - "Investigação dos mecanismos de inibição da resposta de hipersensibilidade tipo I da prole mediada pela imunização materna murina: modulação de citocinas, indução de linfócitos T e B reguladores e influência da natureza do alérgeno."
• Beneficiário: Alberto José da Silva Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular