| Full text | |
| Author(s): |
da Ressureicao Sgnotto, Fabio
;
Garcia de Oliveira, Marilia
;
de Lima Lira, Aline Aparecida
;
Bento-de-Souza, Luciana
;
da Silva Duarte, Alberto Jose
;
Russo Victor, Jefferson
Total Authors: 6
|
| Document type: | Journal article |
| Source: | HUMAN VACCINES & IMMUNOTHERAPEUTICS; v. 13, n. 7, p. 1563-1572, 2017. |
| Web of Science Citations: | 5 |
| Abstract | |
The regulatory effect of allergic responses induced by IgG antibodies on human intra-thymic cells has not been reported in the literature. The aim of this study was to evaluate the possible differential effect of purified IgG from atopic and non-atopic individuals on human intra-thymic T cell cytokine production. Thymic tissues were obtained from 14 patients who were less than 7 d old. Additionally, blood samples were collected from atopic and non-atopic volunteers. Thymocytes and peripheral blood mononuclear cells were cultured with purified atopic or non-atopic IgG, and intracellular cytokine production was assessed. Purified IgG did not influence the frequency or viability of human intra-thymic T cells. Purified non-atopic IgG induced greater IFN-gamma production by intra-thymic CD4+CD8+ T cells than did the mock treatment and atopic IgG. A similar effect of purified non-atopic IgG on TCD8 cells was observed compared with the mock treatment. Atopic IgG inhibited IFN-gamma and TGF-beta production by intra-thymic TCD4 cells. Treatment with intravenous immunoglobulin resulted in intermediate levels of IFN-gamma and TGF-beta in intra-thymic TCD4 cells compared with treatment with atopic and non-atopic IgG. Peripheral TCD4 cells from non-atopic individuals produced IFN-gamma only in response to atopic IgG. This report describes novel evidence revealing that IgG from atopic individuals may influence intracellular IFN-gamma production by intra-thymic T cells in a manner that may favor allergy development. (AU) | |
| FAPESP's process: | 15/17256-3 - "Mechanisms involved on offspring allergy development regulation by maternal immunization: role of cytokines, induction of T and B regulatory cells and allergen nature." |
| Grantee: | Alberto José da Silva Duarte |
| Support Opportunities: | Regular Research Grants |