Our group has investigated the mechanisms involved in the regulation of allergic responses mediated by IgG antibodies. This hypothesis derived from the observation that IgG from mothers immunized with the mite Dermatophagoides pteronyssinus (Der p) is passively transmitted to pups during the gestation period and its detection in offspring serum is directly related to the inhibition of allergies (Victor et al., 2003).In recent years, we have investigated this hypothesis with murine and human approaches, which has demonstrated the modulatory effect of IgG on the maturation of B and T cells in both species. However, a human approach considering the allergen specificity of the IgG donors has not yet been described in the literature. Considering that the most frequent allergy-inducing antigen is provided by the mite Der p, we consider this allergen as a priority in our in investigations. Therefore, this project aims to analyze the effect of IgG from Der p-allergic individuals on the maturation and cytokines production of human lymphocytes with importance in allergy induction and regulation. In this context, we highlight iNKT, TCD4, TCD8, ³dT and B cells, and the following cytokines: IL-4, IFN-³, IL-13, IL-9, IL-10, IL-17, and IL-22. In addition, we intend to evaluate the expression of activation and homing molecules in these cells, including CLA, CCR6, CD25 and a4b7. The results obtained with the implementation of this proposal will elucidate the mechanism of regulation and susceptibility to allergies development collaborating with the future development of immunoregulatory therapies.
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