Analysis of the modulating effect of IgG antibodies from atopic individuals to the mite Dermatophagoides pteronyssiunus on the maturation and cytokine production of human intrathymic lymphocytes

• Grant number: 19/09741-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2019
• End date: August 31, 2020
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Jefferson Russo Victor
• Grantee: Thamires Rodrigues de Sousa
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Our group has investigated the mechanisms involved in the regulation of allergic responses mediated by IgG antibodies. This hypothesis derived from the observation that IgG from mothers immunized with the mite Dermatophagoides pteronyssinus (Der p) is passively transmitted to pups during the gestation period and its detection in offspring serum is directly related to the inhibition of allergies (Victor et al., 2003).In recent years, we have investigated this hypothesis with murine and human approaches, which has demonstrated the modulatory effect of IgG on the maturation of B and T cells in both species. However, a human approach considering the allergen specificity of the IgG donors has not yet been described in the literature. Considering that the most frequent allergy-inducing antigen is provided by the mite Der p, we consider this allergen as a priority in our in investigations. Therefore, this project aims to analyze the effect of IgG from Der p-allergic individuals on the maturation and cytokines production of human lymphocytes with importance in allergy induction and regulation. In this context, we highlight iNKT, TCD4, TCD8, ³dT and B cells, and the following cytokines: IL-4, IFN-³, IL-13, IL-9, IL-10, IL-17, and IL-22. In addition, we intend to evaluate the expression of activation and homing molecules in these cells, including CLA, CCR6, CD25 and a4b7. The results obtained with the implementation of this proposal will elucidate the mechanism of regulation and susceptibility to allergies development collaborating with the future development of immunoregulatory therapies.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FAGUNDES, BEATRIZ OLIVEIRA; DE SOUSA, THAMIRES RODRIGUES; NASCIMENTO, ANDREZZA; FERNANDES, LORENA ABREU; SGNOTTO, FABIO DA RESSUREICAO; ORFALI, RAQUEL LEAO; AOKI, VALERIA; DUARTE, ALBERTO JOSE DA SILVA; SANABANI, SABRI SAEED; VICTOR, JEFFERSON RUSSO. IgG from Adult Atopic Dermatitis (AD) Patients Induces Nonatopic Neonatal Thymic Gamma-Delta T Cells (gamma delta T) to Acquire IL-22/IL-17 Secretion Profile with Skin-Homing Properties and Epigenetic Implications Mediated by miRNA. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 12, p. 16-pg., 2022-06-01. (18/05181-7, 18/08631-3, 19/09741-0)

DE SOUSA, THAMIRES RODRIGUES; FAGUNDES, BEATRIZ OLIVEIRA; NASCIMENTO, ANDREZZA; FERNANDES, LORENA ABREU; SGNOTTO, FABIO DA RESSUREICAO; ORFALI, RAQUEL LEAO; AOKI, VALERIA; DA SILVA DUARTE, ALBERTO JOSE; SANABANI, SABRI SAEED; VICTOR, JEFFERSON RUSSO. IgG from Adult Atopic Dermatitis (AD) Patients Induces Thymic IL-22 Production and CLA Expression on CD4+T Cells: Possible Epigenetic Implications Mediated by miRNA. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 12, p. 18-pg., 2022-06-01. (18/08631-3, 19/09741-0, 18/05181-7)

DE SOUSA, THAMIRES RODRIGUES; VICTOR, JEFFERSON RUSSO. Natural Self-Ligand Gamma Delta T Cell Receptors (gamma delta TCRs) Insight: The Potential of Induced IgG. VACCINES, v. 8, n. 3, SEP 2020. (19/09741-0, 18/05181-7)