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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Protein SUMOylation is Involved in Cell-cycle Progression and Cell Morphology in Giardia lamblia

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Autor(es):
Di Genova, Bruno M. ; da Silva, Richard C. ; da Cunha, Julia P. C. ; Gargantini, Pablo R. ; Mortara, Renato A. ; Tonelli, Renata R.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Journal of Eukaryotic Microbiology; v. 64, n. 4, p. 491-503, JUL-AUG 2017.
Citações Web of Science: 1
Resumo

The unicellular protozoa Giardia lamblia is a food- and waterborne parasite that causes giardiasis. This illness is manifested as acute and self-limited diarrhea and can evolve to long-term complications. Successful establishment of infection by Giardia trophozoites requires adhesion to host cells and colonization of the small intestine, where parasites multiply by mitotic division. The tight binding of trophozoites to host cells occurs by means of the ventral adhesive disc, a spiral array of microtubules and associated proteins such as giardins. In this work we show that knock down of the Small Ubiquitin-like MOdifier (SUMO) results in less adhesive trophzoites, decreased cell proliferation and deep morphological alterations, including at the ventral disc. Consistent with the reduced proliferation, SUMO knocked-down trophozoites were arrested in G1 and in S phases of the cell cycle. Mass spectrometry analysis of anti-SUMO immunoprecipitates was performed to identify SUMO substrates possibly involved in these events. Among the identified SUMOylation targets, -tubulin was further validated by Western blot and confirmed to be a SUMO target in Giardia trophozoites. (AU)

Processo FAPESP: 10/15042-2 - Infecção por Giardia lamblia: busca por moléculas envolvidas na interação parasita-hospedeiro
Beneficiário:Renata Rosito Tonelli
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
Processo FAPESP: 13/04272-5 - O papel da SUMOilação de proteínas na biologia celular de Giardia lamblia e na interação com o hospedeiro
Beneficiário:Bruno Martorelli Di Genova
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto