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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protein SUMOylation is Involved in Cell-cycle Progression and Cell Morphology in Giardia lamblia

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Author(s):
Di Genova, Bruno M. ; da Silva, Richard C. ; da Cunha, Julia P. C. ; Gargantini, Pablo R. ; Mortara, Renato A. ; Tonelli, Renata R.
Total Authors: 6
Document type: Journal article
Source: Journal of Eukaryotic Microbiology; v. 64, n. 4, p. 491-503, JUL-AUG 2017.
Web of Science Citations: 1
Abstract

The unicellular protozoa Giardia lamblia is a food- and waterborne parasite that causes giardiasis. This illness is manifested as acute and self-limited diarrhea and can evolve to long-term complications. Successful establishment of infection by Giardia trophozoites requires adhesion to host cells and colonization of the small intestine, where parasites multiply by mitotic division. The tight binding of trophozoites to host cells occurs by means of the ventral adhesive disc, a spiral array of microtubules and associated proteins such as giardins. In this work we show that knock down of the Small Ubiquitin-like MOdifier (SUMO) results in less adhesive trophzoites, decreased cell proliferation and deep morphological alterations, including at the ventral disc. Consistent with the reduced proliferation, SUMO knocked-down trophozoites were arrested in G1 and in S phases of the cell cycle. Mass spectrometry analysis of anti-SUMO immunoprecipitates was performed to identify SUMO substrates possibly involved in these events. Among the identified SUMOylation targets, -tubulin was further validated by Western blot and confirmed to be a SUMO target in Giardia trophozoites. (AU)

FAPESP's process: 10/15042-2 - Infection by Giardia lamblia: search for molecules involved in parasita-host interaction
Grantee:Renata Rosito Tonelli
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 13/04272-5 - The role of SUMOylation of proteins in cell biology of Giardia lamblia and in the interaction with the host
Grantee:Bruno Martorelli Di Genova
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)