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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Validation of QF-PCR for prenatal diagnoses in a Brazilian population

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Autor(es):
de Moraes, Renata Wendel ; Burlacchini de Carvalho, Mario Henrique ; de Amorim-Filho, Antonio Gomes ; Vieira Francisco, Rossana Pulcineli ; Romao, Renata Moscolini ; Levi, Jose Eduardo ; Zugaib, Marcelo
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Clinics; v. 72, n. 7, p. 400-404, JUL 2017.
Citações Web of Science: 4
Resumo

OBJECTIVES: Quantitative fluorescence polymerase chain reaction (QF-PCR) is a rapid and reliable method for screening aneuploidies, but in Brazil, it is not used in public services. We investigated the accuracy of QF-PCR for the prenatal recognition of common aneuploidies and compared these results with cytogenetic results in our laboratory. METHOD: A ChromoQuant QF-PCR kit containing 24 primer pairs targeting loci on chromosomes 21, 13, 18, X and Y was employed to identify aneuploidies of the referred chromosomes. RESULTS: A total of 162 amniotic fluid samples analyzed using multiplex QF-PCR were compared with karyotyping analysis. The QF-PCR results were consistent with the results of cytogenetic analysis in 95.4% of all samples. CONCLUSION: QF-PCR was demonstrated to be efficient and reliable for prenatal aneuploidy screening. This study suggests that QF-PCR can be used as a rapid diagnostic method. However, rearrangements and some mosaic samples cannot be detected with this test; thus, those exceptions must undergo cytogenetic analysis. (AU)

Processo FAPESP: 12/51806-2 - Implantação e validação de teste QF-PCR para detecção de aneuploidias no ambulatoria de obstetrícia e ginecologia HC FMUSP
Beneficiário:Mario Henrique Burlacchini de Carvalho
Modalidade de apoio: Auxílio à Pesquisa - Pesquisa em Políticas Públicas para o SUS