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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photobiosynthesis of stable and functional silver/silver chloride nanoparticles with hydrolytic activity using hyperthermophilic beta-glucosidases with industrial potential

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Autor(es):
Araujo, Juscemacia N. ; Tofanello, Aryane ; da Silva, Viviam M. ; Sato, Juliana A. P. ; Squina, Fabio M. ; Nantes, Iseli L. ; Garcia, Wanius
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 102, p. 84-91, SEP 2017.
Citações Web of Science: 7
Resumo

The beta-glucosidases are important enzymes employed in a large number of processes and industrial applications, including biofuel production from biomass. Therefore, in this study, we reported for the first time the photobiosynthesis of stable and functional silver/silver chloride nanoparticles (Ag/AgCl-NPs) using two hyperthermostable bacterial beta-glucosidases with industrial potential. The syntheses were straight-forward and rapid processes carried out by mixing beta-glucosidase and silver nitrate (in buffer 10 mM Tris-HCl, pH 8) under irradiation with light (over a wavelength range of 450-600 nm), therefore, compatible with the green chemistry procedure. Synthesized Ag/AgCl-NPs were characterized using a series of physical techniques. Absorption spectroscopy showed a strong absorption band centered at 460 nm due to surface plasmon resonance of the Ag-NPs. X-ray diffraction analysis revealed that the Ag/AgCl-NPs were purely crystalline in nature. Under electron microscopy, Ag/AgCl-NPs of variable diameter ranging from 10 to 100 nm can be visualized. Furthermore, electron microscopy, zeta potential and Fourier transform infrared spectroscopy results confirmed the presence of beta-glucosidases coating and stabilizing the Ag/AgCl-NPs. Finally, the results showed that the enzymatic activities were maintained in the beta-glucosidases assisted Ag/AgCl-NPs. The information described here should provide a useful basis for future studies of beta-glucosidases assisted Ag/AgCl-NPs, including biotechnological applications. (C) 2017 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 15/17688-0 - Nanoestruturas metálicas associadas a porfirinas, citocromo c e azul de metileno: estudos fundamentais para aplicações em sistema biológicos e energia
Beneficiário:Iseli Lourenço Nantes Cardoso
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/02897-3 - Análise estrutural e funcional do domínio fibronectina tipo III (FnIII) de uma beta-glicosidase da família GH3: interação com substratos poliméricos e termoestabilidade
Beneficiário:Wanius José Garcia da Silva
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 14/02065-5 - Modular hipertermoestável endo-beta-1,4-mananase: determinação da estrutura tridimensional do domínio central e seu papel na termoestabilização global da enzima
Beneficiário:Viviam Moura da Silva
Linha de fomento: Bolsas no Brasil - Doutorado