Photobiosynthesis of stable and functional silver/silver chloride nanoparticles with hydrolytic activity using hyperthermophilic beta-glucosidases with industrial potential

Author(s)

Araujo, Juscemacia N. ; Tofanello, Aryane ; da Silva, Viviam M. ; Sato, Juliana A. P. ; Squina, Fabio M. ; Nantes, Iseli L. ; Garcia, Wanius

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 102, p. 84-91, SEP 2017.
• Web of Science Citations: 7

Abstract

The beta-glucosidases are important enzymes employed in a large number of processes and industrial applications, including biofuel production from biomass. Therefore, in this study, we reported for the first time the photobiosynthesis of stable and functional silver/silver chloride nanoparticles (Ag/AgCl-NPs) using two hyperthermostable bacterial beta-glucosidases with industrial potential. The syntheses were straight-forward and rapid processes carried out by mixing beta-glucosidase and silver nitrate (in buffer 10 mM Tris-HCl, pH 8) under irradiation with light (over a wavelength range of 450-600 nm), therefore, compatible with the green chemistry procedure. Synthesized Ag/AgCl-NPs were characterized using a series of physical techniques. Absorption spectroscopy showed a strong absorption band centered at 460 nm due to surface plasmon resonance of the Ag-NPs. X-ray diffraction analysis revealed that the Ag/AgCl-NPs were purely crystalline in nature. Under electron microscopy, Ag/AgCl-NPs of variable diameter ranging from 10 to 100 nm can be visualized. Furthermore, electron microscopy, zeta potential and Fourier transform infrared spectroscopy results confirmed the presence of beta-glucosidases coating and stabilizing the Ag/AgCl-NPs. Finally, the results showed that the enzymatic activities were maintained in the beta-glucosidases assisted Ag/AgCl-NPs. The information described here should provide a useful basis for future studies of beta-glucosidases assisted Ag/AgCl-NPs, including biotechnological applications. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 15/02897-3 - Structural and functional analysis of the fibronectin type III domain (FnIII) of one beta-glucosidase belonging to the family GH3: interaction with large polymeric substrates and thermostability
• Grantee: Wanius José Garcia da Silva
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 14/02065-5 - Modular Hyperthermostable endo-beta-1,4-mannanase: determination of the three-dimensional structure of the Central Domain an its role in the thermostabilization of whole enzyme
• Grantee: Viviam Moura da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/17688-0 - Metallic Nanostructures Associated to Porphyrins, Cytochrome c and Methylene Blue: Fundamental Studies for Applications for Biological Systems and Energy
• Grantee: Iseli Lourenço Nantes Cardoso
• Support Opportunities: Regular Research Grants