Monoolein-alginate beads as a platform to promote adenosine cutaneous localization and wound healing

Autor(es)

Ng, Wing Y. ; Migotto, Amanda ; Ferreira, Thamyres Soares ; Lopes, Luciana B.

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 102, p. 1104-1111, SEP 2017.
• Citações Web of Science: 5

Resumo

Alginate beads containing the polar lipid monoolein were developed as a strategy to manage wet wounds by providing improved uptake of excess exudate while releasing adenosine locally for promotion of healing. To obtain monoolein-containing beads, the lipid was mixed with almond oil (2:1 w/w), and emulsified within the alginate aqueous dispersion, followed by ionotropic gelation in CaCl2 solution. Compared to alginate-only, monoolein-alginate systems were 1.44-fold larger, their swelling ability was 1.40-fold higher and adenosine cumulative release was approximately 1.30-fold lower (at 24h). Monoolein-alginate beads were considered safe for topical application as demonstrated by the absence of changes on the viability of reconstructed skin equivalents compared to PBS. Smaller amounts of adenosine were delivered by the beads into and across damaged porcine skin (created by an incisional wound) compared to the drug aqueous solution, and cutaneous localization was favored. More specifically, the beads increased the viable skin layer/receptor phase delivery ratio by approximately 4-fold at 12 h post-application. Considering the wide range of adenosine physiological effects and the importance of skin localization for its use in wound healing, these results demonstrate the potential of monoolein-containing beads for localized drug delivery and management of wet wounds. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/16617-7 - Sistemas nanoestruturados tópicos para co-localização de agentes quimioterápicos e quimiopreventivos na pele e mama
• Beneficiário: Luciana Biagini Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/23976-9 - Desenvolvimento, caracterização e avaliação de microemulsões para veiculação tópica de paclitaxel e ceramida C6
• Beneficiário: Amanda Migotto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica