Integration host factor is important for biofilm formation by Salmonella enterica Enteritidis

Autor(es)

Leite, Bruna ; Werle, Catierine Hirsch ; do Carmo, Camila Pinheiro ; Nobrega, Diego Borin ; Milanez, Guilherme Paier ; Culler, Hebert Fabricio ; Sircili, Marcelo Palma ; Alvarez-Martinez, Cristina E. ; Brocchi, Marcelo

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS AND DISEASE; v. 75, n. 6 AUG 2017.
• Citações Web of Science: 3

Resumo

Salmonella enterica Enteritidis forms biofilms and survives in agricultural environments, infecting poultry and eggs. Bacteria in biofilms are difficult to eradicate compared to planktonic cells, causing serious problems in industry and public health. In this study, we evaluated the role of ihfA and ihfB in biofilm formation by S. enterica Enteritidis by employing different microbiology techniques. Our data indicate that ihf mutant strains are impaired in biofilm formation, showing a reduction in matrix formation and a decrease in viability and metabolic activity. Phenotypic analysis also showed that deletion of ihf causes a deficiency in curli fimbriae expression, cellulose production and pellicle formation. These results show that integration host factor has an important regulatory role in biofilm formation by S. enterica Enteritidis. (AU)

• Processo FAPESP: 12/10608-3 - PERFIL FENOTÍPICO E GENOTÍPICO DA RESISTÊNCIA AOS ANTIMICROBIANOS EM Klebsiella pneumoniae, Salmonella enterica E ESTAFILOCOCOS COAGULASE-NEGATIVA
• Beneficiário: Diego Borin Nobrega
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/25426-8 - Formação de biofilmes em diferentes mutantes nulos de Salmonella enterica.
• Beneficiário: Bruna Leite Simioni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/05382-6 - Avaliação da virulência e expressão gênica diferencial in vivo de Salmonella enterica mutantes para proteínas associadas ao nucleóide.
• Beneficiário: Guilherme Paier Milanez
• Modalidade de apoio: Bolsas no Brasil - Doutorado