Chemical Composition, Antibacterial, Schistosomicidal, and Cytotoxic Activities of the Essential Oil of Dysphania ambrosioides (L.) Mosyakin & Clemants (Chenopodiaceae)

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Soares, Marina H. ; Dias, Herbert J. ; Vieira, Tatiana M. ; de Souza, Maria G. M. ; Cruz, Ana F. F. ; Badoco, Fernanda R. ; Nicolella, Heloiza D. ; Cunha, Wilson R. ; Groppo, Milton ; Martins, Carlos H. G. ; Tavares, Denise C. ; Magalhaes, Lizandra G. ; Crotti, Antonio E. M.

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRY & BIODIVERSITY; v. 14, n. 8 AUG 2017.
• Citações Web of Science: 7

Resumo

We have investigated the chemical composition and the antibacterial activity of the essential oil of Dysphania ambrosioides (L.) Mosyakin \& Clemants (Chenopodiaceae) (DA-EO) against a representative panel of cariogenic bacteria. We have also assessed the invitro schistosomicidal effects of DA-EO on Schistosoma mansoni and its cytotoxicity to GM07492-A cells invitro. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS) revealed that the monoterpenes cis-piperitone oxide (35.2%), p-cymene (14.5%), isoascaridole (14.1%), and -terpinene (11.6%) were identified by as the major constituents of DA-EO. DA-EO displayed weak activity against Streptococcus sobrinus and Enterococcus faecalis (minimum inhibitory concentration (MIC)=1000g/ml). On the other hand, DA-EO at 25and 12.5g/ml presented remarkable schistosomicidal action invitro and killed 100% of adult worm pairs within 24 and 72h, respectively. The LC50 values of DA-EO were 6.50 +/- 0.38, 3.66 +/- 1.06, and 3.65 +/- 0.76g/ml at 24, 48, and 72h, respectively. However, DA-EO at concentrations higher than 312.5g/ml significantly reduced the viability of GM07492-A cells (IC50=207.1 +/- 4.4g/ml). The selectivity index showed that DA-EO was 31.8 times more toxic to the adult S. mansoni worms than GM07492-A cells. Taken together, these results demonstrate the promising schistosomicidal potential of the essential oil of Dysphania ambrosioides. (AU)

• Processo FAPESP: 07/54241-8 - Prospecção de óleos essenciais de espécies brasileiras cultiváveis com atividade contra patógenos da cavidade bucal
• Beneficiário: Antônio Eduardo Miller Crotti
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores
• Processo FAPESP: 16/19272-9 - Avaliação da atividade antimicrobiana de curcuminóides e estudo de suas reações de fragmentação em fase gasosa por espectrometria de massas sequencial
• Beneficiário: Tatiana Manzini Vieira
• Modalidade de apoio: Bolsas no Brasil - Mestrado