| Full text | |
| Author(s): Show less - |
Soares, Marina H.
;
Dias, Herbert J.
;
Vieira, Tatiana M.
;
de Souza, Maria G. M.
;
Cruz, Ana F. F.
;
Badoco, Fernanda R.
;
Nicolella, Heloiza D.
;
Cunha, Wilson R.
;
Groppo, Milton
;
Martins, Carlos H. G.
;
Tavares, Denise C.
;
Magalhaes, Lizandra G.
;
Crotti, Antonio E. M.
Total Authors: 13
|
| Document type: | Journal article |
| Source: | CHEMISTRY & BIODIVERSITY; v. 14, n. 8 AUG 2017. |
| Web of Science Citations: | 7 |
| Abstract | |
We have investigated the chemical composition and the antibacterial activity of the essential oil of Dysphania ambrosioides (L.) Mosyakin \& Clemants (Chenopodiaceae) (DA-EO) against a representative panel of cariogenic bacteria. We have also assessed the invitro schistosomicidal effects of DA-EO on Schistosoma mansoni and its cytotoxicity to GM07492-A cells invitro. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS) revealed that the monoterpenes cis-piperitone oxide (35.2%), p-cymene (14.5%), isoascaridole (14.1%), and -terpinene (11.6%) were identified by as the major constituents of DA-EO. DA-EO displayed weak activity against Streptococcus sobrinus and Enterococcus faecalis (minimum inhibitory concentration (MIC)=1000g/ml). On the other hand, DA-EO at 25and 12.5g/ml presented remarkable schistosomicidal action invitro and killed 100% of adult worm pairs within 24 and 72h, respectively. The LC50 values of DA-EO were 6.50 +/- 0.38, 3.66 +/- 1.06, and 3.65 +/- 0.76g/ml at 24, 48, and 72h, respectively. However, DA-EO at concentrations higher than 312.5g/ml significantly reduced the viability of GM07492-A cells (IC50=207.1 +/- 4.4g/ml). The selectivity index showed that DA-EO was 31.8 times more toxic to the adult S. mansoni worms than GM07492-A cells. Taken together, these results demonstrate the promising schistosomicidal potential of the essential oil of Dysphania ambrosioides. (AU) | |
| FAPESP's process: | 16/19272-9 - Evaluation of the antimicrobial activity of curcuminoids and study of their fragmentation reactions in the gas-phase by tandem mass spectrometry |
| Grantee: | Tatiana Manzini Vieira |
| Support Opportunities: | Scholarships in Brazil - Master |