Encapsulation of essential oils for use in pharmaceutical compositions with antimicrobial activity

• Grant number: 18/26069-0
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2019 - December 31, 2021
• Field of knowledge: Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
• Principal Investigator: Wanderley Pereira Oliveira
• Grantee: Wanderley Pereira Oliveira
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: André Pitondo da Silva ; Marcia Regina von Zeska Kress ; Mônica Lopes Aguiar
• Associated scholarship(s): 19/25981-0 - Encapsulation of essential oils for use in pharmaceutical formulations with antimicrobial activity, BP.TT

Abstract

Natural compounds have been used in many industrial sectors due to their significant biological properties. In this way, the essential oils have aroused high interest, particularly due to its wide spectrum of proved biological activities, such as antifungal, antibacterial, insecticide, antiviral, antioxidant, among others. The use of essential oils (OEs) as antimicrobial agents has been proposed as an innovative and promising alternative to develop new treatments to overcome microbial resistance. EOs have several active molecules in their composition acting in a synergistic way. This fact, strength the potential of using OEs alone or in association with traditional antibiotics as alternative sources of antimicrobials agents. However, despite the great potential of technological application, OEs present several restrictions, such as chemical complexity, high volatility, susceptibility to degradation and propensity to oxidation, insolubility in aqueous systems, and in general low bioavailability, which difficult to use them directly in more elaborated products. The encapsulation of OEs in micro and nanostructured systems emerges as a powerful strategy to overcome these constraints and, in addition, might positively influence the biological activity of these them, as reported in several studies of literature and from our research group. The process encompasses the inclusion of the active agent in an encapsulating matrix, which may be constituted of carbohydrates, gums, proteins, lipids or other natural or synthetic polymeric materials. These systems can release their contents under specific conditions through a controlled release. In this proposal it will be investigated three different innovative systems for the encapsulation of essential oils, namely, molecular inclusion in cyclodextrins, nanostructured lipid carriers (NLCs), and pro-liposomes. Factors such as the composition of EO, of the encapsulation formulation and the production process influence the physicochemical properties of the engineered product, but this information is scarce in the literature, in a systematized way. Studies of the literature have reported the high potential of OEs as antimicrobial agents and the increase of the antimicrobial activity of these compounds caused by the encapsulation processes, resulting in an increased effectiveness. Thus, the study of the effect of the encapsulation processes on the biological activity of EOs used is also an objective of this proposal. Future researches will be conducted with the aim to evaluate synergism effects between the essential oils and antibiotics linked with the encapsulation process as a strategy to combat the resistance of pathogenic microorganisms. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BANKOLE, VICTOR OLORUNTOBA; OSUNGUNNA, MICHAEL OLUWOLE; FERNANDES SOUZA, CLAUDIA REGINA; SALVADOR, SERGIO LUIZ; OLIVEIRA, WANDERLEY PEREIRA. Spray-Dried Proliposomes: an Innovative Method for Encapsulation of Rosmarinus officinalis L. Polyphenols. AAPS PHARMSCITECH, v. 21, n. 5, MAY 18 2020. (14/15905-1, 18/26069-0, 11/10333-1)

BALDIM, IARA; SOUZA, CLAUDIA R. F.; DURAZZO, ALESSANDRA; LUCARINI, MASSIMO; SANTINI, ANTONELLO; SOUTO, ELIANA B.; OLIVEIRA, WANDERLEY P.. Spray-Dried Structured Lipid Carriers for the Loading of Rosmarinus officinalis: New Nutraceutical and Food Preservative. FOODS, v. 9, n. 8, AUG 2020. (18/26069-0, 11/10333-1)

BALDIM, IARA; PAZIANI, MARIO H.; GRIZANTE BARIAO, PATRICIA H.; KRESS, MARCIA R. VON ZESKA; OLIVEIRA, WANDERLEY P.. anostructured Lipid Carriers Loaded with Lippia sidoides Essential Oil as a Strategy to Combat the Multidrug-Resistant Candida auri. PHARMACEUTICS, v. 14, n. 1, JAN 2022. (14/15905-1, 18/26069-0)

SOUZA, CLAUDIA R. F.; BALDIM, IARA; BANKOLE, VICTOR O.; DA ANA, RAQUEL; DURAZZO, ALESSANDRA; LUCARINI, MASSIMO; CICERO, NICOLA; SANTINI, ANTONELLO; SOUTO, ELIANA B.; OLIVEIRA, WANDERLEY P.. Spouted Bed Dried Rosmarinus officinalis Extract: A Novel Approach for Physicochemical Properties and Antioxidant Activity. AGRICULTURE-BASEL, v. 10, n. 8, AUG 2020. (18/26069-0, 12/03427-2, 11/10333-1)

DA MATA, GUSTAVO CARDOSO; MORAIS, MARIA SIRLENE; DE OLIVEIRA, WANDERLEY PEREIRA; AGUIAR, MONICA LOPES. Sustainable surgical masks: optimizing fine/ultrafine particle filtration using PVA/chitosan electrospun nanofibers. ENVIRONMENTAL SCIENCE-NANO, v. 10, n. 8, p. 16-pg., 2023-07-07. (18/26069-0)

BALDIM, IARA; OLIVEIRA, ANDRESSA M.; SOUTO, ELIANA B.; OLIVEIRA, WANDERLEY P.. yclodextrins-in-Liposomes: A Promising Delivery System for Lippia sidoides and Syzygium aromaticum Essential Oil. LIFE-BASEL, v. 12, n. 1, JAN 2022. (18/26069-0)

OLIVEIRA, WANDERLEY P.; MIGUEL, LUCIANE M.. Encapsulation of volatile oils with antimicrobial activity in chitosan-beta CD molecular inclusion complexes by spray drying. Drying Technology, v. N/A, p. 13-pg., 2022-09-01. (18/26069-0, 14/15905-1)

DA MATA, GUSTAVO CARDOSO; MORAIS, MARIA SIRLENE; DE OLIVEIRA, WANDERLEY PEREIRA; AGUIAR, MONICA LOPES. Composition Effects on the Morphology of PVA/Chitosan Electrospun Nanofibers. POLYMERS, v. 14, n. 22, p. 19-pg., 2022-11-01. (18/26069-0)

BALDIM, IARA; ROSA, DEBORA M.; SOUZA, CLAUDIA R. F.; DA ANA, RAQUEL; DURAZZO, ALESSANDRA; LUCARINI, MASSIMO; SANTINI, ANTONELLO; SOUTO, ELIANA B.; OLIVEIRA, WANDERLEY P.. Factors Affecting the Retention Efficiency and Physicochemical Properties of Spray Dried Lipid Nanoparticles Loaded with Lippia sidoides Essential Oil. BIOMOLECULES, v. 10, n. 5, MAY 2020. (18/26069-0, 11/10333-1)

BALDIM, IARA; OLIVEIRA, WANDERLEY P.; KADIAN, VARSHA; RAO, REKHA; YADAV, NITESH; MAHANT, SHEEFALI; LUCARINI, MASSIMO; DURAZZO, ALESSANDRA; DA ANA, RAQUEL; CAPASSO, RAFFAELE; et al. Natural Ergot Alkaloids in Ocular Pharmacotherapy: Known Molecules for Novel Nanoparticle-Based Delivery Systems. BIOMOLECULES, v. 10, n. 7, JUL 2020. (12/03427-2, 11/10333-1, 18/26069-0)

MORAIS, MARIA S.; BONFIM, DANIELA P. F.; AGUIAR, MONICA L.; OLIVEIRA, WANDERLEY P.. Electrospun Poly (Vinyl Alcohol) Nanofibrous Mat Loaded with Green Propolis Extract, Chitosan and Nystatin as an Innovative Wound Dressing Material. JOURNAL OF PHARMACEUTICAL INNOVATION, v. N/A, p. 15-pg., 2022-08-30. (18/26069-0, 16/20500-6)