Evaluation of the chemical profile and the schistosomicidal potential of the fungal line 1A15 associated with the Ascidia Aplidium accarense

• Grant number: 18/12791-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2018
• Effective date (End): August 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Ana Helena Januário
• Grantee: Thaís de Freitas Alves
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The number of fungal species of marine origin described in recent years has shown a significant increase in the interest of researchers in the study of fungi associated with ascidians. The class Ascidiacea (Ascidians) produces an incredible variety of structurally novel and pharmacologically interesting substances. In this project we intend to carry out the chemical investigation of the methanolic extract of the fungal lineage Trichoderma sp-A15 associated with the invertebrate Aplidium accarense. This species was selected for study within the Regular Research Project "Investigation of the Schistosomicidal, Leishmanicidal and Cytotoxic Potential of Fungal Lineages Associated with Marine Invertebrates Belonging to the Ascidiacea" (Fapesp Process 2017 / 14261-1). The literature reports for the fungal species Trichoderma sp varied activities as phytotoxic, antibacterial, antioxidant and antifungal. However, according to the literature, studies about its schistosomicidal potential are non-existent. Therefore, the chemical approach will be biomonitored for this activity. The schistosomicidal assay will be performed by evaluating the life cycle of the Schistosoma mansoni vector. The analytical methodologies to be used will involve chromatographic separation techniques (CC and CLAE / DAD) and the structural characterization of the isolated compounds will be performed using spectroscopic methods such as uni and bi-dimensional NMR. The main objective of this project will be to identify natural compounds of the marine microbiota with schistosomicidal potential, besides contributing to the formation of human resources in the area of marine natural products in the country.