Synergistic interaction of ten essential oils against Haemonchus contortus in vitro

Autor(es)

Katiki, L. M. ; Barbieri, A. M. E. ; Araujo, R. C. ; Verissimo, C. J. ; Louvandini, H. ; Ferreira, J. F. S.

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 243, p. 47-51, AUG 30 2017.
• Citações Web of Science: 11

Resumo

Anthelmintic resistance in sheep gastrointestinal nematodes is a worldwide problem. Multi-drug resistant haemonchosis is the most serious impediment for small ruminant systems, and there are no new drug candidates currently under development. Molecules from natural sources have demonstrated anthelmintic activity against parasites. In this work, the monoterpenoids carvacrol, carvone, cineole, linalool, limonene, and thymol and the phenylpropanoids cinnamaldehyde, anethole, vanillin, and eugenol were assessed individually or in mixtures of ten binary, three ternary, and three quaternary combinations using the in vitro egg hatch assay with eggs of a multi-drug resistant strain of Haemonchus contortus. The main objective of this study was to identify the most effective interaction among essential oils with the greatest individual anthelmintic efficacy and to determine the most powerful combinations. The essential oils were ranked by their 50% lethal concentration (LC50) as follows (mg/mL): cinamaldehyde (0.018), anethole (0.070), carvone (0.085), carvacrol (0.11), thymol (0.13), linalool (0.29), vanillin (0.57), eugenol (0.57), cineole (4.74), and limonene (207.5). Quantification of synergism, additive effect, and antagonism were calculated for binary, ternary, and quaternary combinations. The best anthelmintic effect resulting from synergistic activity among 16 different combinations was for cinnamaldehyde:carvacrol (CL50 0.012 mg/mL) and anethole:carvone (CL50 0.013 mg/mL). These results indicate that these binary combinations would be promising to be tested in sheep infected with H. contortus. (AU)

• Processo FAPESP: 12/50587-5 - Efeito de óleos essenciais microencapsulados sobre a infecção por Haemonchus contortus, a toxicidade e a imunidade de cordeiros
• Beneficiário: Luciana Morita Katiki
• Modalidade de apoio: Auxílio à Pesquisa - Regular