Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization

Autor(es)

Mancini Coelho, Maria Paula ; Moreira-de-Sousa, Cristina ; de Souza, Raphael Bastao ; Ansoar-Rodriguez, Yadira ; Mathias Silva-Zacarin, Elaine Cristina ; Fontanetti, Carmem Silvia

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 24, n. 27, p. 22007-22017, SEP 2017.
• Citações Web of Science: 5

Resumo

Large amounts of residues generated by agricultural, urban and industrial activities are dumped daily on the soil. This practice deserves special attention because it causes serious environmental problems. This study evaluated the toxic potential of the sugarcane vinasse, a by-product of the sugar-alcohol industry, and the biosolid, a residue produced by wastewater treatment plants, both widely used as fertilizers. The evaluation was performed through bioassays using a typical soil bioindicator, the diplopod Rhinocricus padbergi. The specimens were exposed to soils containing these residues in concentrations that are compatible with the Brazilian regulation for agricultural use. Semi-quantitative immunolabelling analyses of the stress protein HSP70 were performed on the midgut of the studied diplopods. There was a significant increase in the immunolabelling of HSP70 proteins as a response to xenobiotics from both residues, particularly in regions where the function of the cells is the detoxification of the organ (e.g. the hepatic cell layer and specific regions of the epithelium). Higher immunolabelling was observed in the specimens exposed to vinasse in comparison with the biosolid exposure. This demonstrates that the substances in the tested residues had proteotoxic action in the exposed animals and induced a cytoprotective response, which led to higher stress protein immunolabelling. Therefore, caution is needed for the use of such residues in agriculture. (AU)

• Processo FAPESP: 14/12646-5 - Avaliação da toxicidade de amostras de vinhaça e biossólido no intestino médio de diplópodos por meio da marcação de proteínas de estresse
• Beneficiário: Maria Paula Mancini Coelho
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 12/50197-2 - Ação de produtos empregados no cultivo da cana-de-açúcar sobre organismos não alvos
• Beneficiário: Carmem Silvia Fontanetti Christofoletti
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático