Draft genome sequence of an aminoglycoside-resistant RmtD2-producing Enterobacter cloacae subsp cloacae ST395 in Brazil

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Martins, Evelin Rodrigues ; Casella, Tiago ; Campagnari Bueno, Maria Fernanda ; Francisco, Gabriela Rodrigues ; Tolentino, Fernanda Modesto ; Tegon de Freitas, Ana Carolina ; Cerdeira, Louise ; Costa, Monalisa Nogueira ; Cevada, Carla ; Lincopan, Nilton ; Garcia, Doroti de Oliveira ; Lelles Nogueira, Mara Correa

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 10, p. 308-309, SEP 2017.
• Citações Web of Science: 1

Resumo

Objectives: Enterobacter cloacae has recently emerged as an important agent of nosocomial infections owing to the dissemination of extended-spectrum beta-lactamases and carbapenemases in this species. In this context, a rise in the therapeutic use of aminoglycosides was noticed, followed by the accelerated development of resistance mechanisms. In this study, we report the draft genome sequence of a multidrug-resistant E. cloacae subsp. cloacae strain (Ec(2)) isolated from an active surveillance culture of a patient with Chagas disease. Methods: Whole-genome sequencing (WGS) was performed using a Nextera XT DNA library and NextSeq platform. Results: WGS analysis revealed the presence of 5527 coding genes, 62 RNA-encoding genes and 275 pseudogenes. Strain Ec2 belongs to sequence type 395 (ST395) and carries 22 transferable antibiotic resistance genes, comprising eight antimicrobial classes, including the rmtD2 gene conferring high-level aminoglycoside resistance. Conclusions: This draft genome can be used in comparative genomic analyses with different E. cloacae strains. In addition, it could help at elucidating epidemiological aspects regarding the dissemination of clinically relevant resistance genes. (C) 2017 Published by Elsevier Ltd on behalf of International Society for Chemotherapy of Infection and Cancer. (AU)

• Processo FAPESP: 15/21325-0 - Análise comparativa do resistoma de Klebsiella pneumoniae XDR (NDM-1/KPC-2) pertencente ao complexo clonal (CC) endêmico de alto risco CC258
• Beneficiário: Louise Teixeira Cerdeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/17184-0 - Identificação de genes de ESBL, resistência a quinolonas e de virulência em E. coli isoladas de frangos, carne de frango e de pacientes com infecção do trato urinário
• Beneficiário: Mara Corrêa Lelles Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular