Molecular characterization of transport lectin vesicular integral membrane protein 36 kDa (VIP36) in the life cycle of Schistosoma mansoni

Autor(es)

Ornelas, Alice Maria de M. ; de Paula, Renato G. ; Morais, Enyara R. ; Magalhaes, Lizandra G. ; da Silva, Annielle M. B. ; Gomes, Matheus S. ; de Castro-Borges, William ; Rodrigues, Vanderlei

• Tipo de documento: Artigo Científico
• Fonte: Parasitology Research; v. 116, n. 10, p. 2765-2773, OCT 2017.
• Citações Web of Science: 0

Resumo

VIP36 is a protein described as an L-type lectin in animals, responsible for the intracellular transport of glycoproteins within the secretory pathway, and also localized on the plasma membrane. Schistosoma mansoni has a complex system of vesicles and protein transport machinery to the cell surface. The excreted/secreted products of the larvae and eggs are known to be exposed to the host immune system. Hence, characterizing the role and action of SmVIP36 in the S. mansoni life cycle is important for a better understanding of the parasite-host relationship. To this purpose, we firstly performed in silico analysis. Analysis of SmVIP36 in silico revealed that it contains a lectin leg-like domain with a jellyroll fold as seen by its putative 3D tertiary structure. Additionally, it was also observed that its CRD contains calcium ion-binding amino acids, suggesting that the binding of SmVIP36 to glycoproteins is calcium-dependent. Finally, we observed that the SmVIP36 predicted amino acid sequence relative to its orthologs was conserved. However, phylogenetic analysis revealed that SmVIP36 follows species evolution, forming a further cluster with its definitive host Homo sapiens. Moreover, q-PCR analysis in the S. mansoni life cycle points to a significant increase in gene expression in the eggs, schistosomulae, and female adult stages. Similarly, protein expression increased in eggs, cercariae, schistosomulae, and adult worm stages. These results suggest that SmVIP36 might participate in the complex secretory activity within the egg envelope and tegument proteins, both important for the stages of the parasite that interact with the host. (AU)

• Processo FAPESP: 11/04081-0 - Caracterização estrutural e funcional de uma proteína ligadora de manose (Sm60) em Schistosoma mansoni
• Beneficiário: Alice Maria de Magalhães Ornelas
• Modalidade de apoio: Bolsas no Brasil - Mestrado