Establishment of primary mixed cell cultures from spontaneous canine mammary tumors: Characterization of classic and new cancer-associated molecules

Autor(es):Mostrar menos -

Gentile, Luciana B. ^[1] ; Nagamine, Marcia K. ^[1] ; Biondi, Luiz R. ^[1] ; Sanches, Daniel S. ^[1] ; Toyota, Fabio ^[2] ; Giovani, Tatiane M. ^[3] ; de Jesus, Isis P. ^[1] ; da Fonseca, Ivone I. M. ^[1] ; Queiroz-Hazarbassanov, Nicolle ^[4] ; Diaz, Bruno L. ^[5] ; Massoco Salles Gomes, Cristina de O. ^[4] ; Daglil, Maria Lucia Z. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Lab Expt & Comparat Oncol, Sao Paulo - Brazil

^[2] Vet Hosp Caes & Gatos, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Vet Med & Anim Sci, Appl Pharmacol & Toxicol Lab, Sao Paulo - Brazil

^[5] Fed Univ Rio de Janeiro UFRJ, Lab Inflammat, Carlos Chagas Filho Biophys Inst IBCCF, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 12, n. 9 SEP 25 2017.
• Citações Web of Science: 0

Resumo

There are many factors which make canine cancer like cancer in humans. The occurrence of spontaneous mammary tumors in pet dogs, tumor genetics, molecular targets and exposure to the same environmental risk factors are among these factors. Therefore, the study of canine cancer can provide useful information to the oncology field. This study aimed to establish and characterize a panel of primary mixed cell cultures obtained from spontaneous canine mammary tumors. Eight established cell cultures obtained from one normal mammary gland, one complex adenoma, one mixed adenoma, two complex carcinomas and two mixed carcinomas were analyzed. The gene expression levels of classic molecular cancer players such as fibroblast growth factor receptor (FGFR) 2, breast cancer (BRCA) 1, BRCA2 and estrogen receptor (ESR) 1 were evaluated. For the first time, three orphan nuclear receptors, estrogen-related receptors (ERRs) alpha,beta and gamma were studied in canine mammary cancer. The highest expression level of ERR alpha was observed in complex carcinoma-derived cell culture, while the highest levels of ERR beta and gamma were observed in cells derived from a mixed carcinoma. Meanwhile, complex carcinomas presented the highest levels of expression of ESR1, BRCA1 and FGFR2 among all samples. BRCA2 was found exclusively in complex adenoma. The transcription factor GATA3 had its highest levels in mixed carcinoma samples and its lowest levels in complex adenoma. Proliferation assays were also performed to evaluate the mixed cell cultures response to ER ligands, genistein and DES, both in normoxia and hypoxic conditions. Our results demonstrate that morphological and functional studies of primary mixed cell cultures derived from spontaneous canine mammary tumors are possible and provide valuable tool for the study of various stages of mammary cancer development. (AU)

• Processo FAPESP: 11/02400-0 - Caracterização molecular e funcional de receptores relacionados a receptor de estrogênio (ERR)± em neoplasias mamárias espontâneas de cães
• Beneficiário: Luciana Boffoni Gentile
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado