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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Diabetes increases interleukin-17 levels in periapical, hepatic, and renal tissues in rats

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Autor(es):
Azuma, Mariane Maffei [1, 2] ; Gomes-Filho, Joao Eduardo [1] ; Carrara Prieto, Annelise Katrine [1] ; Samuel, Renata Oliveira [1, 3] ; Felix de Lima, Valeria Marcal [4] ; Sumida, Doris Hissako [5] ; Ervolino, Edilson [5] ; Angelo Cintra, Luciano Tavares [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Dept Endodont, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Sao Paulo - Brazil
[2] UNINGA, Inga Univ Ctr, Dept Endodont, Rod PR 317, 6114 Parque Ind 200, Maringa, Parana - Brazil
[3] Univ Uberaba, UNIUBE, Dent Sch, Dept Clin Dent, Av Nene Sabino 1801, BR-38055500 Uberaba, MG - Brazil
[4] Sao Paulo State Univ, UNESP, Dept Clin & Surg & Anim Reprod, Aracatuba Vet Med, Rua Clovis Pestana 793, BR-16050680 Sao Paulo - Brazil
[5] Sao Paulo State Univ, UNESP, Dept Basic Sci, Sch Dent, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 83, p. 230-235, NOV 2017.
Citações Web of Science: 5
Resumo

Objectives: This study aimed to evaluate the association between endodontic infection and diabetes on interleukin-17 levels in periapical, hepatic, and renal tissues of rats. Design: Forty male rats were divided into groups: normoglycemic rats (N), normoglycemic rats with apical periodontitis (N-AP), rats with experimental diabetes (ED), and rats with experimental diabetes and apical periodontitis (ED-AP). Diabetes was induced by intravenous streptozotocin injection, and blood sugar levels were monitored to confirm disease development. Apical periodontitis (AP) was induced by pulp exposure to the oral environment during 30 days. After 30 days, hepatic and renal tissues were obtained, and IL-17 levels were quantified by ELISA. The right hemi-jaw was used to quantify IL-17 levels by immunohistochemistry. The values obtained in parametric tests were tabulated and, analyzed statistically by analysis of variance (ANOVA) and Tukey tests, and the values obtained for scores were statistically analyzed by using the and Dun tests. The level of significance was set at 5%. Results: ED and ED-AP groups expressed significantly higher IL-17 levels in both hepatic and renal tissues (p < 0.05), compared to N and N-AP groups. Apical periodontitis (AP) in ED-AP group was significantly more severe than that in N-AP group (p < 0.05). Furthermore, there was a significantly larger increase in the IL-17 levels in ED-AP group compared to N group (p < 0.05). Conclusion: Our results indicate that diabetes increases IL-17 levels in hepatic and renal tissues and also enhances IL-17 production in apical periodontitis area of rats. (AU)

Processo FAPESP: 12/02565-2 - Influência da infecção pulpar isolada ou associada à diabetes na quantificação da citocina pró-inflamatória IL-17, em lesões periapicais e orgãos de ratos Wistar
Beneficiário:Mariane Maffei Azuma
Linha de fomento: Bolsas no Brasil - Mestrado