Sericin from Bombyx mori cocoons. Part I: Extraction and physicochemical-biological characterization for biopharmaceutical applications

Autor(es):Mostrar menos -

Rocha, Liliam K. H. ^[1] ; Favaro, Laura I. L. ^[1] ; Rios, Alessandra C. ^[1] ; Silva, Erica C. ^[1] ; Silva, Welida F. ^[1] ; Stigliani, Tatiane P. ^[1] ; Gunger, Mariana ^[1] ; Lima, Renata ^[1] ; Oliveira, Jr., Jose M. ^[1] ; Aranha, Norberto ^[1] ; Tubino, Matthieu ^[2] ; Vila, Marta M. D. C. ^[1] ; Balcao, Victor M. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sorocaba, UNISO, PhageLab Lab Biofilms & Bacteriophages, Intelligent Biosensing & Biom Stabilizat Res Grp, Sorocaba, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

^[3] Univ Minho, CEB, Ctr Biol Engn, Braga - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 61, p. 163-177, OCT 2017.
• Citações Web of Science: 8

Resumo

In the present research effort, production of crude sericin extracts from Bombyx mori silk cocoons was attempted using two different approaches. Sericin was extracted from cocoons by high-temperature autoclaving followed either by lyophilization or freezing-thawing precipitation, to obtain a crude sericin powder. The physicochemical and biological characteristics of the crude sericin extracts were evaluated in detail, via FTIR, XRD, XRF, XRT, UV-vis scanning, TGA and DSC, protein quantification, antimicrobial activity, free radical scavenging activity, cytotoxic activity, potential for inducing chromosomal aberrations via Allium cepa assays, and genotoxicity via Comet (TM) analyses. The molecular weight distribution of the crude sericin extracts was also investigated, via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the results duly compared to standard sericin. The results gathered clearly suggest that the crude sericin extracts had both obvious radical scavenging effects with the 2.2-diphenyl-1-picryl-hydrazil (DPPH) assay, and antibacterial activity, further suggesting that this protein might be a valuable addition for either food and biopharmaceutical applications. (AU)

• Processo FAPESP: 16/08884-3 - PneumoPhageColor - desenvolvimento de um kit de biodetecção colorimétrica de Pseudomonas aeruginosa com base em partículas fágicas
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/12234-4 - TransAppIL - permeação cutânea de entidades proteicas estabilizadas estruturalmente e funcionalmente, carreadas por líquidos iônicos
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/16641-3 - Estabilização estrutural e funcional de sericina em hidrogel biopolissacarídico: bio-origami para a regeneração de pele
• Beneficiário: Liliam Katsue Harada Rocha
• Linha de fomento: Bolsas no Brasil - Mestrado