An ultra-high performance liquid chromatography method to determine the skin penetration of an octyl methoxycinnamate-loaded liquid crystalline system

Autor(es)

Prado, A. H. ^[1] ; Borges, M. C. ^[1] ; Eloy, J. O. ^[1] ; Peccinini, R. G. ^[1] ; Chorilli, M. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Sao Paulo UNESP, Coll Pharmaceut Sci, Dept Drugs & Med, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Pharmazie; v. 72, n. 10, p. 563-567, OCT 2017.
• Citações Web of Science: 0

Resumo

Cutaneous penetration is a critical factor in the use of sunscreen, as the compounds should not reach systemic circulation in order to avoid the induction of toxicity. The evaluation of the skin penetration and permeation of the UVB filter octyl methoxycinnamate (OMC) is essential for the development of a successful sunscreen formulation. Liquid-crystalline systems are innovative and potential carriers of OMC, which possess several advantages, including controlled release and protection of the filter from degradation. In this study, a new and effective method was developed using ultra-high performance liquid chromatography (UPLC) with ultraviolet detection (UV) for the quantitative analysis of penetration of OMC-loaded liquid crystalline systems into the skin. The following parameters were assessed in the method: selectivity, linearity, precision, accuracy, robustness, limit of detection (LOD), and limit of quantification (LOQ). The analytical curve was linear in the range from 0.25 to 250 mu g.m(-1), precise, with a standard deviation of 0.05-1.24%, with an accuracy in the range from 96.72 to 105.52%, and robust, with adequate values for the LOD and LOQ of 0.1 and 0.25 mu g.mL(-1), respectively. The method was successfully used to determine the in vitro skin permeation of OMC-loaded liquid crystalline systems. The results of the in vitro tests on Franz cells showed low cutaneous permeation and high retention of the OMC, particularly in the stratum corneum, owing to its high lipophilicity, which is desirable for a sunscreen formulation. (AU)

• Processo FAPESP: 13/00485-4 - Desenvolvimento e caracterização de sistemas líquido-cristalinos contendo p-metoxicinamato de octila: avaliação da permeação e retenção cutânea in vitro
• Beneficiário: Alice Haddad Do Prado
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/22426-2 - Carreadores lipídicos nanoestruturados como potencial estratégia para incorporação do p-metoxicinamato de octila: desenvolvimento, caracterização físico-química e ensaios biológicos in vitro
• Beneficiário: Alice Haddad Do Prado
• Modalidade de apoio: Bolsas no Brasil - Mestrado