Chronic low-dose glucocorticoid treatment increases subcutaneous abdominal fat, but not visceral fat, of male Wistar rats

Autor(es)

Nunes, Patricia Pereira ^[1] ; Andreotti, Sandra ^[1] ; Silva, Flaviane de Fatima ^[1] ; Laurato Sertie, Rogerio Antonio ^[1] ; Caminhotto, Rennan de Oliveira ^[1] ; Medeiros Komino, Ayumi Cristina ^[1] ; Reis, Gabriela Boltes ^[1] ; Lima, Fabio Bessa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 190, p. 29-35, DEC 1 2017.
• Citações Web of Science: 4

Resumo

Aim: Most studies developed to investigate the effects of glucocorticoids chronic treatment on white adipose tissue uses high doses of these hormones. This study analyzes some effects of a chronic, continuous and steady infusion of low-dose hydrocortisone and the relationship with lipid accumulation in white adipose depots in rats. Main methods: Nineteen male Wistar rats were divided into control (CON) and cortisol (CORT) groups. Along six weeks CORT group received continuous infusion of 0.6 mg/kg/day of hydrocortisone, while CON group received saline. After euthanasia, subcutaneous and visceral (retroperitoneal and mesenteric) fat pads were excised, weighted and analyzed for: lipogenic enzymes activity; molecular changes of 11-hydroxysteroid dehydrogenase type 1 (11 beta HSD1) enzyme; enzymes involved in lipid uptake, incorporation, and metabolism and in fatty acids esterification. Besides, morphometric cell analysis was performed. Key findings: CORT group showed increased triglycerides, changes in lipoprotein profile and 26,8% increment in central subcutaneous (SC) mass, while visceral fat pads masses remained unchanged. Adipocytes from SC, only, presented increased fatty acid synthase, ATP-citrate lyase and glucose-6-phosphate dehydrogenase activity, in addition to reduced AMP-activated protein kinase and 11 beta HSD1 enzymes content. Significance: Chronic low-dose hydrocortisone treatment consequences seem to be different from those commonly seen in long term hypercortisolism. While high doses promote lipid accumulation in visceral depots, a low dose showed an increase in central SC depot only. This appears to involve an increment in lipid storage and in de novo lipogenesis enzymes activity. (AU)

• Processo FAPESP: 13/24557-4 - Características da resposta lipogênica diferenciada nos tecidos adiposos de ratos submetidos a uma estimulação excessiva e prolongada com glicocorticóides
• Beneficiário: Patricia Pereira Nunes Traldi
• Modalidade de apoio: Bolsas no Brasil - Mestrado