Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats-part 2: biochemical aspects

Autor(es):Mostrar menos -

Tomazoni, Shaiane Silva ^{[1, 2]} ; Frigo, Lucio ^[3] ; dos Reis Ferreira, Tereza Cristina ^{[4, 5]} ; Casalechi, Heliodora Leao ^[4] ; Teixeira, Simone ^[6] ; de Almeida, Patricia ^[7] ; Muscara, Marcelo Nicolas ^[6] ; Marcos, Rodrigo Labat ^[7] ; Serra, Andrey Jorge ^[7] ; Camillo de Carvalho, Paulo de Tarso ^{[5, 7]} ; Pinto Leal-Junior, Ernesto Cesar ^{[4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Cidade Sao Paulo UNICID, Masters Program Phys Therapy, Rua Cesario Galeno, 448-475, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Cidade Sao Paulo UNICID, Doctoral Program Phys Therapy, Rua Cesario Galeno, 448-475, BR-05508900 Sao Paulo, SP - Brazil

^[3] Cruzeiro do Sul Univ UNICSUL, Biol Sci & Hlth Ctr, Sao Paulo, SP - Brazil

^[4] Nove do Julho Univ UNINOVE, Lab Phototherapy Sports & Exercise, Sao Paulo, SP - Brazil

^[5] Nove do Julho Univ UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo, SP - Brazil

^[7] Nove do Julho Univ UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 32, n. 8, p. 1879-1887, NOV 2017.
• Citações Web of Science: 12

Resumo

Muscle injuries trigger an inflammatory process, releasing important biochemical markers for tissue regeneration. The use of non-steroidal anti-inflammatory drugs (NSAIDs) is the treatment of choice to promote pain relief due to muscle injury. NSAIDs exhibit several adverse effects and their efficacy is questionable. Photobiomodulation therapy (PBMT) has been demonstrated to effectively modulate inflammation induced from musculoskeletal disorders and may be used as an alternative to NSAIDs. Here, we assessed and compared the effects of different doses of PBMT and topical NSAIDs on biochemical parameters during an acute inflammatory process triggered by a controlled model of contusion-induced musculoskeletal injury in rats. Muscle injury was induced by trauma to the anterior tibial muscle of rats. After 1 h, rats were treated with PBMT (830 nm, continuous mode, 100mWof power, 35.71 W/cm(2); 1, 3, and 9 J; 10,30, and 90 s) or diclofenac sodium (1 g). Our results demonstrated that PBMT, 1 J (35.7 J/cm(2)), 3 J (107.1 J/cm(2)), and 9 J (321.4 J/cm(2)) reduced the expression of tumor necrosis factor alpha (TNF-alpha) and cyclooxygenase-2 (COX-2) genes at all assessed times as compared to the injury and diclofenac groups (p < 0.05). The diclofenac group showed reduced levels of COX-2 only in relation to the injury group (p < 0.05). COX-2 protein expression remained unchanged with all therapies except with PBMT at a 3-J dose at 12 h (p < 0.05 compared to the injury group). In addition, PBMT (1, 3, and 9 J) effectively reduced levels of cytokines TNF-alpha, interleukin (IL)-1 beta, and IL-6 at all assessed times as compared to the injury and diclofenac groups (p < 0.05). Thus, PBMTat a 3-J dose was more effective than other doses of PBMT and topical NSAIDs in the modulation of the inflammatory process caused by muscle contusion injuries. (AU)

• Processo FAPESP: 12/06832-5 - Laserterapia de baixa potência e diclofenaco sódico de uso tópico na lesão muscular induzida por contusão em ratos
• Beneficiário: Lúcio Frigo
• Modalidade de apoio: Auxílio à Pesquisa - Regular