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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents

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Autor(es):
Andre, Carolina Bosso [1] ; Rosalen, Pedro Luiz [2] ; Galvao, Livia Camara de Carualho [3, 4] ; Fronza, Bruna Mann [1] ; Boui Ambrosano, Glaucia Maria [5] ; Ferracane, Jack Liborio [6] ; Giannini, Marcelo [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[3] Dom Bosco Grad Sch, Dept Dent, Sao Luis, MA - Brazil
[4] CEUMA Univ, Sao Luis, MA - Brazil
[5] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Social Dent, Piracicaba, SP - Brazil
[6] Oregon Hlth & Sci Univ, Sch Dent, Dept Restorat Dent, Portland, OR 97201 - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Dental Materials; v. 33, n. 10, p. 1084-1092, OCT 2017.
Citações Web of Science: 5
Resumo

Objectives.The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Methods.Apigenin (1 mM) and tt-Farnesol (5 mM) were added separately and in combination to a self-etch adhesive (CS3 Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT OptiBond S).Biofilm of S.mutans was grown on adhesive-coated hydroxyapatite disks for 115 h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Results.Addition of Apigenin and Apigenin + tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S.mutans biofilm.Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT.Intracellular polysaccharide decreased with addition of Apigenin and Apigenin+ tt-Farnesol to CS3.No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Signcance.Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S.mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd.All rights reserved. (AU)

Processo FAPESP: 13/22823-9 - Influência da incorporação de produtos naturais nas propriedades mecânicas e antimicrobiana de sistemas adesivos
Beneficiário:Carolina Bosso André
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/17543-0 - Incorporação de produtos naturais que alteram a virulência do Streptococcus mutans em materiais restauradores
Beneficiário:Carolina Bosso André
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado