Minimum sample sizes for population genomics: an empirical study from an Amazonian plant species

Autor(es)

Nazareno, Alison G. ^[1] ; Bemmels, Jordan B. ^[2] ; Dick, Christopher W. ^[2] ; Lohmann, Lucia G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bot, Rua Matao 277, Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 - USA

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR ECOLOGY RESOURCES; v. 17, n. 6, p. 1136-1147, NOV 2017.
• Citações Web of Science: 45

Resumo

High-throughput DNA sequencing facilitates the analysis of large portions of the genome in nonmodel organisms, ensuring high accuracy of population genetic parameters. However, empirical studies evaluating the appropriate sample size for these kinds of studies are still scarce. In this study, we use double-digest restriction-associated DNA sequencing (ddRADseq) to recover thousands of single nucleotide polymorphisms (SNPs) for two physically isolated populations of Amphirrhox longifolia (Violaceae), a nonmodel plant species for which no reference genome is available. We used resampling techniques to construct simulated populations with a random subset of individuals and SNPs to determine how many individuals and biallelic markers should be sampled for accurate estimates of intra- and interpopulation genetic diversity. We identified 3646 and 4900 polymorphic SNPs for the two populations of A. longifolia, respectively. Our simulations show that, overall, a sample size greater than eight individuals has little impact on estimates of genetic diversity within A. longifolia populations, when 1000 SNPs or higher are used. Our results also show that even at a very small sample size (i.e. two individuals), accurate estimates of F-ST can be obtained with a large number of SNPs (1500). These results highlight the potential of high-throughput genomic sequencing approaches to address questions related to evolutionary biology in nonmodel organisms. Furthermore, our findings also provide insights into the optimization of sampling strategies in the era of population genomics. (AU)

• Processo FAPESP: 15/07141-4 - Testando a hipótese dos rios como barreira biogeográfica para espécies de plantas da Amazônia
• Beneficiário: Alison Gonçalves Nazareno
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/12633-8 - Filogeografia comparada de plantas na Amazônia Central
• Beneficiário: Alison Gonçalves Nazareno
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/50260-6 - Estruturação e evolução da biota amazônica e seu ambiente: uma abordagem integrativa
• Beneficiário: Lúcia Garcez Lohmann
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático