The Drosophila melanogaster Eip74EF-PA transcription factor directly binds the sciarid BhC4-1 promoter

Autor(es)

Frank, Henrique Oliveira ^[1] ; Sanchez, Danilo Garcia ^[2] ; Oliveira, Lucas de Freitas ^[2] ; Kobarg, Jorg ^{[3, 4]} ; Monesi, Nadia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Programa Posgrad Biol Celular & Mol, Fac Med Ribeirao Preto, Av Bandeirantes 3900, BR-14049900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe, BR-14040903 Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Fac Ciencias Farmaceut, Rua Monteiro Lobato 255, BR-13083970 Sao Paulo - Brazil

^[4] Univ Estadual Campinas, Dept Bioquim & Biol Tecidual, Inst Biol, Rua Monteiro Lobato 255, BR-13083970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENESIS; v. 55, n. 11 NOV 2017.
• Citações Web of Science: 1

Resumo

The DNA puff BhC4-1 gene of Bradysia hygida (Diptera, Sciaridae) is amplified and expressed in the salivary glands at the end of the last larval instar. Even though there are no BhC4-1 orthologs in Drosophila melanogaster, the mechanisms that regulate BhC4-1 gene expression in B. hygida are for the most part conserved in D. melanogaster. The BhC4-1 promoter contains a 129bp (-186/-58) cis-regulatory module (CRM) that drives developmentally regulated expression in transgenic salivary glands at the onset of metamorphosis. Both in the sciarid and in transgenic D. melanogaster, BhC4-1 gene expression is induced by the increase in ecdysone titers that triggers metamorphosis. Genetic interaction experiments revealed that in the absence of the Eip74EF-PA early gene isoform BhC4-1-lacZ levels of expression in the salivary gland are severely reduced. Here we show that the overexpression of the Eip74EF-PA transcription factor is sufficient to anticipate BhC4-1-lacZ expression in transgenic D. melanogaster. Through yeast one-hybrid assays we confirm that the Eip74EF-PA transcription factor directly binds to the 129 bp sciarid CRM. Together, these results contribute to the characterization of an insect CRM and indicate that the ecdysone gene regulatory network that promotes metamorphosis is conserved between D. melanogaster and the sciarid B. hygida. (AU)

• Processo FAPESP: 11/50962-8 - Caracterizacao molecular da interacao de fatores trans-ativadores de d. melanogaster com elementos cis-reguladores identificados no promotor do gene de pufe de dna bhc4-1.
• Beneficiário: Nadia Monesi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/24005-9 - Ensaios de trans-ativação em levedura para análise da interação entre o fator de transcrição E74A de D. melanogaster com a região regulatória dos CGs 7599 e 7336 de D. melanogaster
• Beneficiário: Danilo Garcia Sanchez
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica