The Drosophila melanogaster Eip74EF-PA transcription factor directly binds the sciarid BhC4-1 promoter

Author(s)

Frank, Henrique Oliveira ^[1] ; Sanchez, Danilo Garcia ^[2] ; Oliveira, Lucas de Freitas ^[2] ; Kobarg, Jorg ^{[3, 4]} ; Monesi, Nadia ^[2]

Affiliation

^[1] Univ Sao Paulo, Programa Posgrad Biol Celular & Mol, Fac Med Ribeirao Preto, Av Bandeirantes 3900, BR-14049900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe, BR-14040903 Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Fac Ciencias Farmaceut, Rua Monteiro Lobato 255, BR-13083970 Sao Paulo - Brazil

^[4] Univ Estadual Campinas, Dept Bioquim & Biol Tecidual, Inst Biol, Rua Monteiro Lobato 255, BR-13083970 Sao Paulo - Brazil

• Document type: Journal article
• Source: GENESIS; v. 55, n. 11 NOV 2017.
• Web of Science Citations: 1

Abstract

The DNA puff BhC4-1 gene of Bradysia hygida (Diptera, Sciaridae) is amplified and expressed in the salivary glands at the end of the last larval instar. Even though there are no BhC4-1 orthologs in Drosophila melanogaster, the mechanisms that regulate BhC4-1 gene expression in B. hygida are for the most part conserved in D. melanogaster. The BhC4-1 promoter contains a 129bp (-186/-58) cis-regulatory module (CRM) that drives developmentally regulated expression in transgenic salivary glands at the onset of metamorphosis. Both in the sciarid and in transgenic D. melanogaster, BhC4-1 gene expression is induced by the increase in ecdysone titers that triggers metamorphosis. Genetic interaction experiments revealed that in the absence of the Eip74EF-PA early gene isoform BhC4-1-lacZ levels of expression in the salivary gland are severely reduced. Here we show that the overexpression of the Eip74EF-PA transcription factor is sufficient to anticipate BhC4-1-lacZ expression in transgenic D. melanogaster. Through yeast one-hybrid assays we confirm that the Eip74EF-PA transcription factor directly binds to the 129 bp sciarid CRM. Together, these results contribute to the characterization of an insect CRM and indicate that the ecdysone gene regulatory network that promotes metamorphosis is conserved between D. melanogaster and the sciarid B. hygida. (AU)

• FAPESP's process: 12/24005-9 - Analysis of the interaction between the E74A transcription factor and the regulatory region of the D. melanogaster CGs 7599 and 7336 using trans-activating assays in yeast
• Grantee: Danilo Garcia Sanchez
• Support Opportunities: Scholarships in Brazil - Scientific Initiation